Sensitive Analysis of Protein Adsorption to Colloidal Gold by Differential Centrifugal Sedimentation

Analytical Chemistry
Adam M DavidsonMartin Volk

Abstract

It is demonstrated that the adsorption of bovine serum albumin (BSA) to aqueous gold colloids can be quantified with molecular resolution by differential centrifugal sedimentation (DCS). This method separates colloidal particles of comparable density by mass. When proteins adsorb to the nanoparticles, both their mass and their effective density change, which strongly affects the sedimentation time. A straightforward analysis allows quantification of the adsorbed layer. Most importantly, unlike many other methods, DCS can be used to detect chemisorbed proteins ("hard corona") as well as physisorbed proteins ("soft corona"). The results for BSA on gold colloid nanoparticles can be modeled in terms of Langmuir-type adsorption isotherms (Hill model). The effects of surface modification with small thiol-PEG ligands on protein adsorption are also demonstrated.

References

Nov 1, 1987·The Journal of Histochemistry and Cytochemistry : Official Journal of the Histochemistry Society·C De RoeP Baudhuin
Jan 1, 1985·Advances in Protein Chemistry·T Peters
Feb 1, 1996·Journal of Molecular Graphics·W HumphreyK Schulten
Apr 18, 2002·Proceedings of the National Academy of Sciences of the United States of America·Franci Merzel, Jeremy C Smith
Jan 22, 2004·Journal of the American Chemical Society·Rui HongVincent M Rotello
Sep 25, 2004·Protein Science : a Publication of the Protein Society·Hannes FischerAldo F Craievich
Sep 21, 2005·Langmuir : the ACS Journal of Surfaces and Colloids·Scott H BrewerStefan Franzen
Oct 17, 2006·The European Physical Journal. E, Soft Matter·J H TeichroebL W Jones
Feb 3, 2007·Proceedings of the National Academy of Sciences of the United States of America·Tommy CedervallSara Linse
Apr 27, 2007·Analytical Chemistry·Wolfgang HaissDavid G Fernig
Sep 3, 2008·Chemical Society Reviews·Robert Wilson
Dec 4, 2008·Fundamental & Clinical Pharmacology·Sylvain GoutellePascal Maire
Feb 12, 2009·ACS Nano·Jennifer A JamisonVicki L Colvin
Sep 8, 2009·Nature Nanotechnology·Carlheinz RöckerG Ulrich Nienhaus
Apr 2, 2010·Journal of the American Chemical Society·Dorota WalczykKenneth A Dawson
Jun 18, 2010·ACS Nano·Eudald CasalsVictor Puntes
Feb 4, 2011·Journal of the American Chemical Society·Marco P MonopoliKenneth A Dawson
May 20, 2011·Langmuir : the ACS Journal of Surfaces and Colloids·Soumyananda ChakrabortyPinak Chakrabarti
Aug 25, 2011·ACS Nano·Martin LundqvistKenneth Dawson
Oct 18, 2011·Beilstein Journal of Nanotechnology·Pauline MaffreG Ulrich Nienhaus
Apr 21, 2012·Langmuir : the ACS Journal of Surfaces and Colloids·Sergio Dominguez-MedinaStephan Link
Apr 21, 2012·Journal of Controlled Release : Official Journal of the Controlled Release Society·Eugene MahonKenneth A Dawson
Sep 21, 2012·Acta Crystallographica. Section D, Biological Crystallography·Anna Bujacz
Feb 20, 2013·Chembiochem : a European Journal of Chemical Biology·Morteza MahmoudiKenneth A Dawson
Jun 20, 2014·Physical Chemistry Chemical Physics : PCCP·L TreuelM Maskos
Jul 12, 2014·Accounts of Chemical Research·Candace C Fleischer, Christine K Payne
Mar 31, 2015·Nature Nanotechnology·Philip M KellyKenneth A Dawson

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Citations

Jul 14, 2017·Langmuir : the ACS Journal of Surfaces and Colloids·Jordan M DennisonCatherine J Murphy
Aug 8, 2018·Wiley Interdisciplinary Reviews. Nanomedicine and Nanobiotechnology·Bin Li, Lucas A Lane
Mar 14, 2019·Nanoscale·Seth Richard PriceSandor Balog
Jan 22, 2020·Nanoscale·Ali Akbar AshkarranMorteza Mahmoudi
Jan 18, 2020·Neural Computation·Zhenghang CuiMasashi Sugiyama
Nov 24, 2018·Journal of Colloid and Interface Science·Jing WangMatt Trau
Jan 22, 2021·The Journal of Physical Chemistry. B·Nurul Ain AzmanJames Chen Yong Kah
Feb 23, 2021·Nano Letters·Yuxiu ChuChangyou Zhan
Jan 29, 2021·ChemMedChem·Marta GozziEvamarie Hey-Hawkins
Apr 4, 2021·Nanomaterials·Rafaela García-Álvarez, María Vallet-Regí
Apr 14, 2021·Biomedicine & Pharmacotherapy = Biomédecine & Pharmacothérapie·Wei HuangWeiping Min
May 1, 2021·Nanomaterials·Claudia Simone PlüischAlexander Wittemann
Mar 8, 2019·Bioconjugate Chemistry·Guadalupe RuizJeremy D Driskell
Jul 25, 2019·Langmuir : the ACS Journal of Surfaces and Colloids·Guadalupe RuizJeremy D Driskell
Jul 21, 2020·Langmuir : the ACS Journal of Surfaces and Colloids·Olatunde AwotundeJeremy D Driskell

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Methods Mentioned

BETA
transmission
X-ray
fluorescence correlation spectroscopy
FCS

Software Mentioned

Microcal Origin
VMD

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