Sensitive and rapid detection of pathogenic bacteria from urine samples using multiplex recombinase polymerase amplification

Lab on a Chip
Junge ChenWanli Xing

Abstract

Bacterial infections may cause severe diseases such as tuberculosis, sepsis, nephritis and cystitis. The rapid and sensitive detection of bacteria is a prerequisite for the treatment of these diseases. The current gold standard for bacterial identification is bacteriological culture. However, culture-based identification takes 3-7 days, which is time-consuming and laborious. In this study, bacteria in urine samples were enriched using a portable filter-based pipette. Then, a centrifugal chip was constructed to detect multiple pathogenic bacteria from urine samples by integrating the DNA extraction, multiplex recombinase polymerase amplification (RPA) and fluorescent detection together. This eliminated the time-consuming cultivation step, and thus accelerated the diagnosis of the urinary tract infections (UTIs). The five major pathogenic bacteria in UTIs were detected in this study, which are Escherichia coli, Proteus mirabilis, Pseudomonas aeruginosa, Staphylococcus aureus and Salmonella typhimurium. Escherichia coli, Proteus mirabilis, Pseudomonas aeruginosa and Staphylococcus aureus were successfully detected with limits of detection of 100 CFU mL-1 from urine samples within 40 min. Salmonella typhimurium was successfully det...Continue Reading

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Citations

Feb 1, 2020·Foodborne Pathogens and Disease·Bin WangShaopeng Gu
Nov 10, 2018·Analytical Chemistry·Yanling SongChaoyong Yang

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Methods Mentioned

BETA
PCR
nucleic acid amplification
chips
chip
genotyping
nucleic acid analysis

Software Mentioned

Primer
LabDisk

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