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Sensitive and robust detection of citrus greening (huanglongbing) bacterium "Candidatus Liberibacter asiaticus" by DNA amplification with new 16S rDNA-specific primers

Molecular and Cellular Probes

Oct 1, 2012

Takashi Fujikawa, Toru Iwanami

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Abstract

Citrus greening disease is caused by "Candidatus Liberibacter spp.," including "Candidatus Liberibacter asiaticus (Las)." For detecting this disease, we designed new primers from the Las 16S rDNA and used a very small DNA template for PCR. More Las-infected tissues were detected with ou...read more

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  • Sensitive and robust detection of citrus greening (huanglongbing) bacterium "Candidatus Liberibacter asiaticus" by DNA amplification with new 16S rDNA-specific primers

    Molecular and Cellular Probes

    Oct 1, 2012

    Takashi Fujikawa, Toru Iwanami

    PMID: 22728344

    DOI: 10.1016/j.mcp.2012.06.001

    Abstract

    Citrus greening disease is caused by "Candidatus Liberibacter spp.," including "Candidatus Liberibacter asiaticus (Las)." For detecting this disease, we designed new primers from the Las 16S rDNA and used a very small DNA template for PCR. More Las-infected tissues were detected with ou...read more

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    57

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