Sensitive and selective Affimer-functionalised interdigitated electrode-based capacitive biosensor for Her4 protein tumour biomarker detection

Biosensors & Bioelectronics
Pavel ZhurauskiPedro Estrela

Abstract

A novel Affimer-functionalised interdigitated electrode-based capacitive biosensor platform was developed for detection and estimation of Her4, a protein tumour biomarker, in undiluted serum. An anti-Her4 Affimer with a C-terminal cysteine was used to create the bio-recognition layer via self-assembly on gold interdigitated electrodes for the sensor fabrication. Electrochemical impedance spectroscopy (EIS) in the absence of redox markers was used to evaluate the sensor performance by monitoring the changes in capacitance. The Affimer sensor in buffer and in undiluted serum demonstrated high sensitivity with a broad dynamic range from 1 pM to 100 nM and a limit of detection lower than 1 pM both in buffer and in serum. Furthermore, the Affimer sensor demonstrated excellent specificity with negligible interference from serum proteins, suggesting resilience to non-specific binding. The sensing ability of the present Affimer sensor in spiked undiluted serum suggests its potential for a new range of Affimer-based sensors. The fabricated Affimer sensor can thus be further adapted with other probes having affinities to other biomarkers for a new range of biosensors.

Citations

Jun 7, 2018·Angewandte Chemie·Thomas SchlichthaerleRalf Jungmann
Jun 13, 2020·Annual Review of Analytical Chemistry·Prosper KanyongJason J Davis
Aug 14, 2020·Analytical Methods : Advancing Methods and Applications·Hugo José Nogueira Pedroza Dias MelloMarcelo Mulato
May 19, 2019·Scientific Reports·Emma L HeskethNeil A Ranson
Apr 5, 2020·Medical Microbiology and Immunology·J Leva-BuenoP A Millner
Jan 14, 2021·Scientific Reports·Shazana Hilda ShamsuddinPaul A Millner
Oct 31, 2020·Research : a Science Partner Journal·Ruitao LiuTianhong Cui
Feb 13, 2021·Molecules : a Journal of Synthetic Chemistry and Natural Product Chemistry·Mika HatadaKoji Sode
Jan 29, 2021·Biosensors & Bioelectronics·Shazana Hilda ShamsuddinPaul A Millner
Feb 13, 2021·Trends in Biotechnology·Ioannis PrattisLuigi G Occhipinti
Jul 4, 2021·Talanta·Bassam Bachour JuniorMarcelo Mulato

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