Sensitive and simultaneous quantitation of 6β-hydroxycortisol and cortisol in human plasma by LC-MS/MS coupled with stable isotope dilution method

Journal of Mass Spectrometry : JMS
Ryohei HiranoHiromi Shibasaki

Abstract

CYP3A phenotyping provides a means for personalized drug therapy. We focused our attention on the plasma 6β-hydroxycortisol (6β-OHF) to cortisol ratio as an index for CYP3A phenotyping. In the present study, we developed a sensitive and reliable method for the simultaneous determination of 6β-OHF and cortisol in human plasma using high-performance liquid chromatography/tandem mass spectrometry together with picolinylester derivatization or nonderivatization methods and 6β-[9,11,12,12-2 H4 ]hydroxycortisol and [1,2,4,19-13 C4 ]cortisol as internal standards for in vivo CYP3A phenotyping in humans. The lower limits of quantification were 38.513 pg/mL for 6β-OHF and 38.100 pg/mL for cortisol. The relative error and relative standard deviation of the lower limits of quantification were <5% for both methods. The intra-day and inter-day assay reproducibilities of the determined 6β-OHF and cortisol concentrations were consistent with the actual amounts added as relative errors and relative standard deviations for both methods, which were <5.4% and <3.9%, respectively. Both methods were applied for the quantification of plasma 6β-OHF and cortisol concentrations in healthy subjects taking oral contraceptives. The absolute concentrations...Continue Reading

References

Aug 1, 1994·Pharmacogenetics·P B Watkins
Oct 1, 1996·Journal of Pharmacokinetics and Biopharmaceutics·G R Wilkinson
Feb 10, 2000·International Journal of Clinical Pharmacology and Therapeutics·M ZaiglerU Fuhr
Apr 25, 2000·Journal of Chromatography. B, Biomedical Sciences and Applications·T FurutaY Kasuya
Aug 22, 2003·Hormone and Metabolic Research = Hormon- Und Stoffwechselforschung = Hormones Et Métabolisme·I VargaK Rácz
Sep 5, 2003·Journal of Chromatography. B, Analytical Technologies in the Biomedical and Life Sciences·Atsushi SuzukiTakashi Furuta
Oct 23, 2003·Drug Metabolism and Disposition : the Biological Fate of Chemicals·Takashi FurutaYasuji Kasuya
Jun 9, 2005·Journal of Chromatography. B, Analytical Technologies in the Biomedical and Life Sciences·Yu Chen BarrettOle Vesterqvist
Dec 5, 2009·Journal of Chromatography. B, Analytical Technologies in the Biomedical and Life Sciences·Ursula LutzWerner K Lutz
Nov 3, 2012·British Journal of Clinical Pharmacology·Yvonne Mårde ArrhénUlf Diczfalusy
Jan 30, 2015·Journal of Chromatographic Science·Liyun ZhengZeneng Cheng

❮ Previous
Next ❯

Citations

Feb 24, 2021·European Journal of Drug Metabolism and Pharmacokinetics·Akitomo YokokawaHiromi Shibasaki
Oct 19, 2020·Steroids·Bojidarka Ivanova, Michael Spiteller

❮ Previous
Next ❯

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.