Sensitive and specific detection of α-synuclein in human plasma

Journal of Neuroscience Research
R B TinsleyM K Horne

Abstract

alpha-Synuclein (alphasyn) mutations, overexpression, misfolding, and aggregation are associated with Parkinson's disease. This protein has been intensively studied in neuronal systems. However, alphasyn is also present in extracellular fluids, such as cerebrospinal fluid and blood plasma. Recent studies have attempted to quantify its levels and compare these in various extracellular fluids of control and Parkinson's disease subjects. Data from these studies have been difficult to interpret, suggesting that more sensitive, standardized, and well-characterized assays of larger cohorts are required. Here, we describe the development of a new ELISA specifically for quantifying alphasyn in human plasma. An initial assay, using a commercial anti-alphasyn monoclonal antibody (211; Santa Cruz Biotechnology, Santa Cruz, CA) and based on a published protocol, was adapted for use in human plasma. In addition, we have developed a novel alphasyn-specific antibody for the assay that has very high sensitivity and signal:noise characteristics. Assays with either antibody showed high specificity for alphasyn, and detected it in a variety of sample types, including plasma. These assays can now be employed on large cohorts of patients and contro...Continue Reading

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Citations

Jan 10, 2013·PloS One·Ana GorostidiOmar M A El-Agnaf
Apr 7, 2015·PloS One·Niklas K U KoehlerElke Richartz-Salzburger
Nov 24, 2019·Movement Disorders : Official Journal of the Movement Disorder Society·Sireesha ManneAnumantha G Kanthasamy
Oct 11, 2015·Journal of Neurochemistry·Brit MollenhauerMichael G Schlossmacher
Jul 17, 2018·Molecular Neurobiology·Thein Than HtikeBalázs Gulyás

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