PMID: 11328583May 1, 2001Paper

Sensitive and specific immunological detection methods for porcine endogenous retroviruses applicable to experimental and clinical xenotransplantation

Xenotransplantation
Stefan J TackeJoachim Denner

Abstract

The use of organs from transgenic pigs for xenotransplantation may be associated with the risk of transmission of microorganisms, especially when the transgenic pigs express human proteins influencing complement activation. The porcine endogenous retroviruses (PERVs) are of particular concern as they can infect human cells in vitro. However, it is unknown whether PERVs can infect transplant recipients in vivo and, if so, whether they are pathogenic. It is therefore essential for experimental and clinical xenotransplantation procedures that specific and sensitive screening methods for PERVs are established. We developed Western blot and enzyme-linked immunosorbant assays (ELISA) based on purified PERVs produced by pig and human cells or recombinant viral protein and synthetic peptides corresponding to PERVs' transmembrane envelope protein, respectively. PERV-specific anti-sera generated against purified virus particles, purified viral proteins and synthetic peptides served as positive controls. Both assays were used for screening the sera of healthy blood donors, pregnant women, patients treated with pig tissues, and butchers with extensive contact to living porcine material to detect antibodies against PERV. None of the individ...Continue Reading

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Citations

Sep 6, 2003·Journal of Clinical Virology : the Official Publication of the Pan American Society for Clinical Virology·Markus IrgangJoachim Denner
Dec 7, 2006·Proceedings of the National Academy of Sciences of the United States of America·Anna AronovichYair Reisner
Apr 12, 2012·Clinical Microbiology Reviews·Joachim Denner, Ralf R Tönjes
Nov 6, 2004·Journal of Clinical Microbiology·O GarkavenkoR B Elliott
May 16, 2006·Journal of Virology·Uwe FiebigJoachim Denner
Jun 14, 2006·PLoS Medicine·Smadar Eventov-FriedmanYair Reisner
Dec 10, 2008·Expert Opinion on Biological Therapy·C G Thanos, R B Elliott
Jun 17, 2008·Transplantation Proceedings·E DörrschuckR R Tönjes
Feb 19, 2008·Comparative Immunology, Microbiology and Infectious Diseases·Bjoern PetersenHeiner Niemann
May 16, 2014·Viruses·Magdalena C KimsaUrszula Mazurek
Sep 26, 2003·Transplantation·Debra A MacKenzieHans W Sollinger
Oct 30, 2004·Annals of Medicine·Roumiana S Boneva, Thomas M Folks
Nov 8, 2007·Reviews in Medical Virology·Derrick LouzRob C Hoeben
May 3, 2005·Transplant International : Official Journal of the European Society for Organ Transplantation·Monica E WinklerUlrich Martin
Jan 5, 2011·Archives of Virology·D KaulitzJ Denner
Jan 14, 2004·The Journal of General Virology·Takele ArgawCarolyn A Wilson
Apr 19, 2020·Xenotransplantation·Joachim Denner
Apr 14, 2012·Xenotransplantation·Marwan SemaanJoachim Denner
Apr 14, 2012·Xenotransplantation·Jay A FishmanYasuhiro Takeuchi
Jun 13, 2002·Xenotransplantation·Jürgen H BluschUlrich Martin
Aug 30, 2002·Xenotransplantation·Mark B NottleChristopher G Grupen

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