PMID: 8943759Oct 1, 1996Paper

Sensitive and universal method for microbial DNA extraction from blood products

Journal of Clinical Pathology
N GolbangP Williamson

Abstract

A new method of extracting bacterial and yeast DNA from blood products dependent on guanidinium thiocyanate acid extraction and proteinase K treatment is described. In spiked samples the sensitivity per 0.1 ml of serum and blood, respectively, was 26 and 150 colony forming units (cfu) for Escherichia coli, 80 and 120 cfu for Staphylococcus aureus and 20 and 26 cfu for Candida albicans. This compared well with existing methodologies, worked on limited clinical samples and was not pathogen specific.

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Citations

May 1, 1997·European Journal of Clinical Microbiology & Infectious Diseases : Official Publication of the European Society of Clinical Microbiology·J P BurnieR C Matthews
Mar 23, 2002·The Journal of Antimicrobial Chemotherapy·S M WigfieldJ P Burnie
Aug 26, 1998·European Journal of Clinical Microbiology & Infectious Diseases : Official Publication of the European Society of Clinical Microbiology·B E LeyM R Millar
Apr 29, 2004·Clinical Microbiology and Infection : the Official Publication of the European Society of Clinical Microbiology and Infectious Diseases·A HeiningerG Döering
Dec 3, 2014·Clinical Chemistry·Stephanie A Thatcher
Aug 17, 2021·Journal of Clinical Laboratory Analysis·Xiao-Xia ChenSu-Mei Cao

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