Sensitive colorimetric detection of K(I) using catalytically active gold nanoparticles triggered signal amplification

Biosensors & Bioelectronics
Zhengbo ChenYonghui Li

Abstract

In this work, we report a simple, ultrasensitive, and feasible colorimetric assay for metal ion (K(+), used as a model) via inherent peroxidase-like enzymatic amplification strategy of gold nanoparticles (AuNPs). It is shown that peroxidase-like activity of AuNPs can be improved dramatically by its surface activation with target-specific aptamer molecules. Whereas when the target exists, the aptamers leave the surface of AuNPs in a target concentration-dependent manner, resulting in a decrease of the nanoenzymatic catalytic ability of AuNPs. Thus, K(+) can be quantified in the presence of AuNPs by using a colorimetric sensing probe (3,3',5,5'-tetramethylbenzidine). The color change of the solution is relevant to the dose of the target, and this can be achieved with the naked eyes and monitored by UV-vis spectrometry. A linear dependence between the absorbance and target K(+) concentration is obtained under optimal conditions in the range from 0. 1 nM to 1 μM with a detection limit (LOD) of 0.06 nM estimated at the 3Sblank level. The sensitivity displays to be 2-9 orders of magnitude better than those of other K(+) detection methods. This sensing strategy may in principle be universally applicable for the detection of a range of...Continue Reading

References

Aug 30, 1990·Nature·A D Ellington, J W Szostak
Jun 19, 2004·Journal of Toxicology and Environmental Health. Part B, Critical Reviews·Elena CraftMohamed Abou-Donia
Mar 3, 2012·Chemphyschem : a European Journal of Chemical Physics and Physical Chemistry·Sheng WangXin-Hua Lin

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Citations

Sep 30, 2020·Analytical Methods : Advancing Methods and Applications·Yangyang ChangJuewen Liu
Dec 20, 2020·Biosensors & Bioelectronics·Bankuru NavyathaSeema Nara
Jan 29, 2021·The Analyst·Renzhong YuZhihui Dai
Jul 18, 2021·Analytical Biochemistry·Rezvan Yazdian-RobatiSeyed Mohammad Taghdisi

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