Sensitive detection and serovar differentiation of typhoidal and nontyphoidal Salmonella enterica species using 16S rRNA Gene PCR coupled with high-resolution melt analysis

The Journal of Molecular Diagnostics : JMD
Billie J MasekCharlotte A Gaydos

Abstract

Salmonella enterica species infections are a significant public health problem causing high morbidity rates worldwide and high mortality rates in the developing world. These infections are not always rapidly diagnosed as a cause of bloodstream infections because of the limitations of blood culture, which greatly affects clinical care as a result of treatment delays. A molecular diagnostic assay that could rapidly detect and identify S. enterica species infections as a cause of sepsis is needed. Nine typhoidal and nontyphoidal S. enterica serovars were used to establish the limit of detection (LOD) of a previously published 16S rRNA gene PCR (16S PCR) in mock whole blood specimens. In addition, 16 typhoidal and nontyphoidal S. enterica serovars were used to evaluate the serovar differentiation capability of 16S PCR coupled with high-resolution melt analysis. The overall LOD of 16S PCR for the nine typhoidal and nontyphoidal S. enterica serovars analyzed was <10 colony-forming units per milliliter (CFU/mL) in mock whole blood specimens, with the lowest and highest LOD at <1 CFU/mL and 9 CFU/mL, respectively. By high-resolution melt analysis, the typhoidal and nontyphoidal S. enterica serovar groups analyzed each generated a uniqu...Continue Reading

References

Jan 19, 2005·The Journal of Infectious Diseases·Jay K VarmaFrederick J Angulo
Feb 29, 2008·Journal of Clinical Microbiology·Samuel YangRichard E Rothman
Dec 18, 2009·Clinical Infectious Diseases : an Official Publication of the Infectious Diseases Society of America·John A Crump, Eric D Mintz
Feb 18, 2010·Clinical Infectious Diseases : an Official Publication of the Infectious Diseases Society of America·Shannon E MajowiczUNKNOWN International Collaboration on Enteric Disease 'Burden of Illness' Studies
Jul 27, 2010·Academic Emergency Medicine : Official Journal of the Society for Academic Emergency Medicine·Richard RothmanCharlotte Gaydos
Jun 23, 2011·Expert Review of Anti-infective Therapy·Christopher M ParryStephen Baker
Nov 29, 2011·Travel Medicine and Infectious Disease·Flor M Sánchez-VargasOscar G Gómez-Duarte
Dec 30, 2011·Journal of Clinical Microbiology·Kevin JengRichard E Rothman
May 18, 2012·Clinical Infectious Diseases : an Official Publication of the Infectious Diseases Society of America·Alicia B CronquistBarbara E Mahon
May 18, 2012·Clinical Infectious Diseases : an Official Publication of the Infectious Diseases Society of America·Shua J ChaiBarbara E Mahon
May 30, 2012·Critical Reviews in Microbiology·Ahmet Koluman, Abdullah Dikici
Jan 29, 2013·Microbial Drug Resistance : MDR : Mechanisms, Epidemiology, and Disease·LaShanda M GlennJonathan G Frye

❮ Previous
Next ❯

Citations

Sep 9, 2016·Scientific Reports·Ki Soo ParkRichelle C Charles
Feb 7, 2017·Scientific Reports·Nadya AndiniSamuel Yang
Apr 3, 2019·Annual Review of Analytical Chemistry·Dong Jin ShinTza-Huei Wang

❮ Previous
Next ❯

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.