Abstract
Azoarcus tolulyticus is a new class of widely distributed toluene-degrading denitrifiers of potential importance in remediating benzene, toluene, ethylbenzene, and xylene (BTEX)-contaminated environments. To detect these organisms in the environment, 16S rRNA gene-based phylogenetic probes were developed. Two sets of specific PCR amplification primers and two oligonucleotide hybridization probes were designed and tested against both closely and distantly related environmental isolates. All of these primers and probes were specific to the species A. tolulyticus. The sensitivity of the PCR amplification primer sets was evaluated with DNA isolated from A. tolulyticus Tol-4 pure culture and from sterile soils seeded with a known number of Tol-4 and Escherichia coli cells. These primer sets were able to detect 1 fg to 1 pg of template DNA from the pure culture and 1.11 x 10(2) to 1.1 x 10(8) Tol-4 cells per g of soil in the presence of 1.56 x 10(10) E. coli cells. These two PCR amplification primers were also successfully tested at two field sites. The primers identified the A. tolulyticus strains among the toluene-degrading bacteria isolated from a low-O2-high-NO(3)- aquifer at Moffett Field, Calif. Also, the presence of A. tolulyt...Continue Reading
References
Jul 1, 1992·Applied and Environmental Microbiology·Y L Tsai, B H Olson
Feb 1, 1992·Applied and Environmental Microbiology·Y L Tsai, B H Olson
Jul 11, 1991·Nucleic Acids Research·R T D'AquilaJ C Kaplan
Nov 11, 1989·Nucleic Acids Research·W Rychlik, R E Rhoads
May 18, 1989·Nature·S Kwok, R Higuchi
Jan 11, 1984·Nucleic Acids Research·J DevereuxO Smithies
May 15, 1981·Journal of Molecular Biology·J BrosiusH F Noller
Sep 1, 1994·Nucleic Acids Research·B L MaidakC R Woese
Apr 1, 1995·International Journal of Systematic Bacteriology·H J AndersG Fuchs
Oct 1, 1995·Molecular Ecology·J Z Zhou, J M Tiedje
Jul 1, 1993·Journal of Clinical Microbiology·J B MahonyM A Chernesky
Apr 1, 1994·Applied and Environmental Microbiology·S M ThiemJ M Tiedje
Aug 1, 1994·Applied and Environmental Microbiology·M R FriesJ M Tiedje
Jul 1, 1993·Nucleic Acids Research·N LarsenC R Woese
Jul 1, 1995·International Journal of Systematic Bacteriology·J ZhouJ M Tiedje
Feb 1, 1996·Applied and Environmental Microbiology·J ZhouJ M Tiedje
Mar 1, 1996·Applied and Environmental Microbiology·J C Chee-SanfordJ M Tiedje
Apr 1, 1997·Applied and Environmental Microbiology·M R FriesJ M Tiedje
Citations
Jul 1, 2004·FEMS Microbiology Ecology·Rainer U MeckenstockChristian Griebler
May 2, 2000·Applied and Environmental Microbiology·G BrakerJ M Tiedje
Sep 8, 2006·Applied Microbiology and Biotechnology·Yoh TakahataKazuya Watanabe
Jun 6, 2003·Applied and Environmental Microbiology·Xueduan LiuJizhong Zhou
Jan 9, 2004·Applied and Environmental Microbiology·Blaz StresJames M Tiedje
Dec 14, 2011·Applied and Environmental Microbiology·Weimin Sun, Alison M Cupples
Dec 3, 1998·Applied and Environmental Microbiology·N M DuTeauK F Reardon
Apr 18, 1998·Applied and Environmental Microbiology·K WatanabeS Harayama
Apr 11, 2018·Chemistry : a European Journal·Julia S LohmannPeter Spiteller
May 29, 2002·Microbial Ecology·S.T.-L. TayM.F. Polz
Jan 6, 2021·International Journal of Phytoremediation·Michael BenIsraelKari E Dunfield
Oct 6, 1998·Applied and Environmental Microbiology·G BrakerK P Witzel