Sensitive detection of Bacillus thuringiensis Cry1B toxin based on camel single-domain antibodies

MicrobiologyOpen
Wenjing ZhongYakun Wan

Abstract

Bt Cry1B toxin, a residue in insect-resistant transgenic plants, has been identified to be harmful to human health. Therefore, it is urgent to detect the Cry1B toxin level in each kind of transgenic plant. Nbs, with prominently unique physiochemical properties, are becoming more and more promising tools in the detection of target antigens. In this study, an immune phage display library that was of high quality was successfully constructed for the screening of Cry1B-specific Nbs with excellent specificity, affinity, and thermostable. Subsequently, a novel sandwich ELISA for Cry1B detection was established, which was based on the biotin-streptavidin system using these aforementioned Nbs. This established detection system presented a linear working range from 5 to 1000 ng ml-1 and a low detection limit of 3.46 ng ml-1 . The recoveries from spiked samples were in the range of 82.51%-113.56% with a relative standard deviation (RSD) lower than 5.00%. Taken together, the proposed sandwich ELISA would be a potential method for the detection of Cry1B toxin in transgenic Bt plants specifically and sensitively.

References

Sep 8, 1998·Microbiology and Molecular Biology Reviews : MMBR·N CrickmoreD H Dean
Aug 24, 1999·Current Topics in Microbiology and Immunology·K Johnsson, L Ge
Jun 11, 2002·Journal of Invertebrate Pathology·Charani Ranasinghe, Raymond J Akhurst
Aug 29, 2003·The Plant Journal : for Cell and Molecular Biology·Anthony J ConnerJan-Peter Nap
Sep 10, 2005·Applied and Environmental Microbiology·Jaroslaw LetowskiLuke Masson
Sep 26, 2006·Drug Discovery Today·Xianghua Yan, Zirong Xu
Apr 3, 2012·Microbial Biotechnology·Alejandra BravoMario Soberón
Feb 11, 2014·Current Protocols in Immunology·Toya Nath BaralMehdi Arbabi Ghahroudi
Mar 1, 2014·Theranostics·Rubel ChakravartyWeibo Cai
May 30, 2014·Plant Methods·Alessandra RocchettiVerena Kriechbaumer
Dec 3, 2014·Toxicon : Official Journal of the International Society on Toxinology·Pingyan WangYakun Wan
Aug 9, 2016·International Journal of Nanomedicine·Yongzhong WangLi Yu
Sep 30, 2016·Journal of Agricultural and Food Chemistry·Yuanyuan LiuQinghua He

❮ Previous
Next ❯

Citations

Oct 11, 2020·Plant Molecular Biology·Wenyi WangChangan Jiang
Jul 27, 2021·Frontiers in Cellular and Infection Microbiology·Kristian Daniel Ralph RothMichael Hust
Jul 29, 2021·Journal of Agricultural and Food Chemistry·Aroosha FaheemYonggang Hu

❮ Previous
Next ❯

Methods Mentioned

BETA
transgenic
ELISA
phage display
PCR
chip
phage
electrophoresis
surface plasmon resonance

Software Mentioned

SPRi
Plexera
Light Cycler

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.