Sensitive Detection of Exosomal Proteins via a Compact Surface Plasmon Resonance Biosensor for Cancer Diagnosis.

ACS Sensors
Chang LiuYun Wu

Abstract

Exosomes are small extracellular vesicles released by cells for cell-cell communication. They play important roles in cancer development, metastasis, and drug resistance. Exosomal proteins have been demonstrated by many studies as promising biomarkers for cancer screening, diagnosis, and monitoring. Among many detection techniques, surface plasmon resonance (SPR) is a highly sensitive, label-free, and real-time optical detection method. Commercial prism-based wavelength/angular-modulated SPR sensors afford high sensitivity and resolution, but their large footprint and high cost limit their adaptability for clinical settings. Recently, a nanoplasmonic exosome (nPLEX) assay was developed to detect exosomal proteins for ovarian cancer diagnosis. However, comparing with conventional SPR biosensors, the broad applications of nanoplasmonic biosensors are limited by the difficult and expensive fabrication of nanostructures. We have developed an intensity-modulated, compact SPR biosensor (25 cm × 10 cm × 25 cm) which uses a conventional SPR sensing mechanism and does not require nanostructure fabrication. Calibration from glycerol showed that the compact SPR biosensor offered sensitivity of 9.258 × 103%/RIU and resolution of 8.311 × 10...Continue Reading

References

Nov 12, 2005·Journal of Industrial Microbiology & Biotechnology·Scott D SoelbergClement E Furlong
Mar 13, 2010·Molecular & Cellular Proteomics : MCP·Joanne L WeltonAled Clayton
May 28, 2013·Cancer Metastasis Reviews·Asfar S AzmiFazlul H Sarkar
Apr 23, 2014·Nature Biotechnology·Hyungsoon ImHakho Lee
May 23, 2014·Analytical Chemistry·Déborah L M RupertFredrik Höök
Jun 25, 2014·Current Opinion in Cell Biology·Joanna KowalClotilde Théry
Nov 19, 2014·Nature Reviews. Urology·Muhammad NawazThomas Kislinger
Mar 5, 2015·Journal of Extracellular Vesicles·Kristine R JakobsenMalene M Jørgensen
Mar 25, 2015·Scientific Reports·Onur TokelUtkan Demirci
May 1, 2015·Analytical and Bioanalytical Chemistry·Luigino GrassoHorst Vogel
May 6, 2015·Expert Review of Molecular Diagnostics·Hyungsoon ImHakho Lee
May 17, 2015·Journal of Extracellular Vesicles·María Yáñez-MóOlivier De Wever
Aug 11, 2015·Scientific Reports·Yun LiuWei Peng
Sep 20, 2012·The Journal of Physical Chemistry Letters·Giuseppe Spoto, Maria Minunni
Jun 28, 2016·Journal of Thoracic Oncology : Official Publication of the International Association for the Study of Lung Cancer·Birgitte Sandfeld-PaulsenBoe Sandahl Sorensen
Jul 29, 2016·Scientific Reports·Abu Ali Ibn SinaMatt Trau
Nov 20, 2016·Molecular Oncology·B Sandfeld-PaulsenB S Sorensen
Jun 21, 2017·Sensors·Xiao-Ling ZhangJun Yang
Aug 31, 2017·Molecular Cancer·Weihua LiDexi Chen

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Citations

Feb 6, 2019·Analytical and Bioanalytical Chemistry·Abu Ali Ibn SinaMatt Trau
Aug 27, 2019·Frontiers in Chemistry·Noemi BellassaiGiuseppe Spoto
Sep 16, 2020·Analytical Methods : Advancing Methods and Applications·Houman Kholafazad Kordasht, Mohammad Hasanzadeh
Dec 7, 2019·Journal of Extracellular Vesicles·Shin La ShuMarc S Ernstoff
Jan 28, 2021·Molecules : a Journal of Synthetic Chemistry and Natural Product Chemistry·Jonalyn DeCastroKiana Aran
Nov 13, 2020·Frontiers in Cell and Developmental Biology·Kaijian ZhouGuoxin Liang
Feb 27, 2021·Analytical and Bioanalytical Chemistry·Cristina Muñoz-San MartínSusana Campuzano
Apr 27, 2021·Bioactive Materials·Komal AbhangeYuan Wan
Apr 23, 2021·Advanced Drug Delivery Reviews·Alice GualerziMarzia Bedoni
Jul 23, 2021·Expert Review of Molecular Diagnostics·Beata OlejnikMirosława Ferens-Sieczkowska
Nov 27, 2021·Journal of Cellular Physiology·Seyed Z RasihashemiParisa Lotfinejad

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