PMID: 8609198Nov 1, 1995Paper

Sensitive detection of group A rotaviruses by immunomagnetic separation and reverse transcription-polymerase chain reaction

Journal of Virological Methods
B GrindeH Ushijima

Abstract

An immunomagnetic separation (IMS) method was developed for concentrating rotaviruses from environmental samples, as well as a reverse transcription-polymerase chain reaction (RT-PCR) for the sensitive and specific detection of group A rotaviruses. Magnetic beads were coated with monoclonal antibodies directed against the group-specific, inner capsid protein (VP6) and subsequently used to capture and purify the virus with the help of a magnet. The genome was made available for RT by heat-disrupting the viral particles. A single 40-cycle PCR was as sensitive as a nested PCR, both detecting 0.005 PFU of the Wa strain, corresponding to approximately 5 particles as indicated by EM. The nested PCR was positive for all the group A strains tested, but negative for group C rotaviruses and other RNA viruses. The IMS-RT-PCR method functioned satisfactorily with virus seeded out in fresh water samples; with sea water, the IMS removed most, but not all, inhibiting activity.

References

Jan 1, 1990·Journal of Virological Methods·L XuM A McCrae
Jul 1, 1986·Archives of Disease in Childhood·H UshijimaT Shinozaki
Jan 11, 1980·Science·R G WyattR M Chanock
Apr 1, 1995·Journal of Virological Methods·T O JonassenB Grinde

❮ Previous
Next ❯

Citations

May 15, 2012·Analytical and Bioanalytical Chemistry·Ivo SafarikMirka Safarikova
Aug 29, 2000·Journal of Virological Methods·S BidawidS A Sattar
Oct 8, 1998·Journal of Virological Methods·C R BarardiK L Williams
Sep 7, 1999·International Journal of Food Microbiology·C R BarardiK L Williams
Aug 17, 2000·Journal of Applied Microbiology·C D ChapronA B Margolin
Feb 20, 2002·Journal of Applied Microbiology·A P Wyn-Jones, J Sellwood
May 11, 2002·Journal of Veterinary Medicine. B, Infectious Diseases and Veterinary Public Health·M ElschnerK Sachse
Mar 29, 2012·The Journal of Infectious Diseases·Joseph S BreseeUNKNOWN US Acute Gastroenteritis Etiology Study Team
Jun 12, 2003·The Pediatric Infectious Disease Journal·Johannes KehleGisela Enders
Aug 2, 2011·Emerging Infectious Diseases·Aron J HallMarc-Alain Widdowson
May 3, 2014·SpringerPlus·Martin M NyagaM Jeffrey Mphahlele
Sep 17, 2011·International Journal of Hygiene and Environmental Health·Ibrahim Ahmed HamzaMichael Wilhelm
Sep 12, 2008·The Journal of Pediatrics·Whitney H LymanJan Vinjé
Aug 19, 2014·Journal of Clinical Virology : the Official Publication of the Pan American Society for Clinical Virology·Oskar SkogOlle Korsgren
Nov 1, 1996·Journal of Medical Virology·K Stene-Johansen, B Grinde
Dec 5, 2016·BMC Pediatrics·Joshua R FrancisChristopher C Blyth
Feb 17, 1998·Applied and Environmental Microbiology·N JothikumarT W Mariam

❮ Previous
Next ❯

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.