PMID: 9545686Apr 18, 1998Paper

Sensitive detection of Helicobacter pylori in gastric aspirates by polymerase chain reaction

Kansenshōgaku zasshi. The Journal of the Japanese Association for Infectious Diseases
R UribeM Nasu

Abstract

The detection of Helicobacter pylori in gastric aspirate was examined by using the polymerase chain reaction (PCR) method for amplifying a specific fragment of the urease gene A. The ability of PCR to amplify H. pylori-specific DNA was analyzed by Southern hybridization with an internal oligonucleotide probe. Twenty-two H. pylori strains from clinical isolates and reference strains were studied, and all H. pylori strains yielded a 356-bp product that hybridized with the oligonucleotide probe, whereas no amplification was evident with 18 non-H. pylori strains. This could detect as little as 50 CFU of H. pylori in pure culture and 0.1 pg of purified chromosomal DNA. A total of 50 dyspeptic patients were examined for the presence of H. pylori by culture, the rapid urease test and histological examination of antral biopsy samples as well as by PCR in gastric juice aspirate samples. The gold standard for the presence of H. pylori was established by minimum concordance of two of three tests performed on biopsy specimens. With this gold standard, 34 of the 50 patients were considered to harbor H. pylori infection. PCR correctly identified 32 (94.1%) of these 34 infected patients. PCR had the best combination of sensitivity and specifi...Continue Reading

Citations

Jan 1, 2014·International Journal of Clinical Practice·M XiaoY Wang
Jan 15, 2014·BMC Gastroenterology·Mariana Ferreira LealMarília Cardoso Smith
Aug 4, 2012·PloS One·Mariana Ferreira LealMarília de Arruda Cardoso Smith
Oct 1, 2016·Scandinavian Journal of Gastroenterology·Floriana GiorgioAlfredo Di Leo
Oct 4, 2014·World Journal of Gastroenterology : WJG·Saurabh Kumar PatelGopal Nath

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