Sensitive detection of intracellular microRNA based on a flowerlike vector with catalytic hairpin assembly

Chemical Communications : Chem Comm
Jintong LiuJianping Lei

Abstract

A flowerlike nanovector is developed for the fluorescence imaging and detection of miRNA in live cells with catalytic hairpin assembly. This horned vector provides a high loading efficiency of DNA amplifiers for multiple signal outputs and is applied to detect miRNA-21 in an average single HeLa cell, showing promising application in trace detection of intracellular biomolecules.

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Citations

Aug 29, 2018·Chemical Communications : Chem Comm·Jiaoli WangKemin Wang
Feb 2, 2019·Angewandte Chemie·Zhihe QingRonghua Yang
May 18, 2019·Chemical Communications : Chem Comm·Lana ZouFuan Wang
Jul 5, 2019·Advanced Materials·Devleena SamantaChad A Mirkin
Sep 18, 2020·Chemical Communications : Chem Comm·Keke GongFuan Wang
Dec 21, 2018·Journal of Materials Chemistry. B, Materials for Biology and Medicine·Dinggeng HeHung-Wing Li
Jul 14, 2021·Chemical Reviews·Min LiQiang Xia
Feb 5, 2019·Chemical Reviews·Friedrich C SimmelHari R Singh

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Methods Mentioned

BETA
transfection
fluorescence imaging
FRET
transmission electron microscopy
dynamic light scattering
electrophoresis
Fluorescence

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