PMID: 2105895Jan 1, 1990Paper

Sensitive detection of Mycoplasma pulmonis by using the polymerase chain reaction

Jikken dobutsu. Experimental animals
S KunitaN Kagiyama

Abstract

Detection of Mycoplasma pulmonis was examined by using the polymerase chain reaction (PCR) for amplifying a specific DNA sequence. In gel electrophoresis which was conducted to detect the amplified products, only 1 pg of M. pulmonis DNA could be detected following 30 cycles of amplification, while no amplified product was detected even from 1 microgram of M. arthritidis or M. neurolyticum DNA. Furthermore, 10 colony-forming units of M. pulmonis could be detected by direct amplification from the mycoplasma suspension. These results suggest the usefulness of the PCR as a highly sensitive, specific, and rapid method for direct detection of M. pulmonis.

Citations

Aug 12, 2005·Experimental Animals·Dong-Jae KimJae-Hak Park

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