Sensitive detection of tumour cells in effusions by combining cytology and fluorescence in situ hybridisation (FISH)

British Journal of Cancer
M FieglG Gastl

Abstract

Diagnosis of malignant cells in effusions is important for staging procedures and resulting therapeutic decisions. Cytodiagnostics in effusions is sometimes difficult since reactive mesothelial cells can mimic malignant cells. We used fluorescence in situ hybridisation (FISH) in single-colour or if appropriate in dual-colour evaluation to detect chromosomal aberrations in effusion cells as markers of malignancy, to raise the diagnostic yield. Cytologic and FISH evaluations--by using probes representing several chromosomes always including chromosomes 11 and 17--were performed in 358 effusion fluids. Cytology was positive for malignancy in 44.4% of all effusions, whereas FISH was positive in 53.9% (P=0.0001). The combination of cytology and FISH was diagnostic for malignancy in 60.9% of effusions. Diagnostic superiority of FISH was demonstrated in effusions from breast cancer, lung cancer, pancreatic cancer, and in effusions from the entire group of gynaecological and gastrointestinal carcinomas. In transudates (effusion protein <2.5 g dl(-1)), malignant cells were detectable by cytology, FISH, and combined use of both methods in 18.6, 30, and 37.1% of effusions, respectively, suggesting that cytologic and molecular analysis sho...Continue Reading

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Citations

Oct 7, 2004·Cytometry. Part B, Clinical Cytometry·Anita MassonerMichael Fiegl
Jun 2, 2005·Current Opinion in Pulmonary Medicine·Michael Fiegl
Oct 22, 2005·Diagnostic Cytopathology·Jareer KassisElise C Kohn
Feb 9, 2008·Supportive Care in Cancer : Official Journal of the Multinational Association of Supportive Care in Cancer·Siyamek Neragi-Miandoab
Dec 22, 2007·Surgical Laparoscopy, Endoscopy & Percutaneous Techniques·Francesco PalazzoMurray J Cohen
Oct 22, 2009·Cytopathology : Official Journal of the British Society for Clinical Cytology·S A ThiryayiP S Hasleton
Dec 14, 2011·Nature Reviews. Clinical Oncology·Frederic W Grannis
Oct 10, 2020·Technology in Cancer Research & Treatment·Kunpeng DuJiqiang Li
Oct 24, 2007·Molecular Cancer Research : MCR·Neveen SaidKouros Motamed
Mar 8, 2006·Archives of Pathology & Laboratory Medicine·Alberto M Marchevsky
Apr 25, 2019·Acta Cytologica·Gordon H YuKaren S Gustafson

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Methods Mentioned

BETA
chromosomal aberrations
flow cytometry
lavage

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