Abstract
Escherichia coli O157:H7, a major foodborne pathogen, is a major public health concern associated with life-threatening diseases such as hemolytic uremic syndrome. To alleviate this burden, a sensitive and rapid system is required to detect this pathogen in various kinds of foods. Herein, we propose a phage-based pathogen detection method to replace laborious and time-consuming conventional methods. We engineered an E. coli O157:H7-specific phage phiV10 to rapidly and sensitively detect this notorious pathogen. The luxCDABE operon was introduced into the phiV10 genome and allowed the engineered phage phiV10lux to generate bioluminescence proportional to the number of viable E. coli O157:H7 cells without any substrate addition. The phage phiV10lux was able to detect at least 1CFU/ml of E. coli O157:H7 in a pure culture within 40min after 5h of pre-incubation. In artificially contaminated romaine lettuce, apple juice (pH3.51), and ground beef, the reporter phage could detect approximately 10CFU/cm(2), 13CFU/ml, and 17CFU/g of E. coli O157:H7, respectively. Taken together, the constructed reporter phage phiV10lux could be applied as a powerful tool for rapid and sensitive detection of live E. coli O157:H7 in foods.
References
Sep 1, 1992·International Journal of Food Microbiology·L RossenO F Rasmussen
Jan 1, 1991·Epidemiologic Reviews·P M Griffin, R V Tauxe
May 1, 1986·Journal of Clinical Microbiology·S B March, S Ratnam
Nov 1, 1995·Journal of Bacteriology·M J Loessner, S Scherer
Aug 1, 1997·Applied and Environmental Microbiology·M J LoessnerS Scherer
Jun 4, 1999·Epidemiology and Infection·J TuttleP M Griffin
Jan 6, 2000·FEMS Microbiology Letters·T E Waddell, C Poppe
Jun 1, 2000·Proceedings of the National Academy of Sciences of the United States of America·K A Datsenko, B L Wanner
Dec 21, 2000·Journal of Applied Microbiology·H ChartM J Woodward
Jan 9, 2004·Applied and Environmental Microbiology·Masahito OdaYasunori Tanji
Apr 28, 2004·Plasmid·Nadège PhilippeDominique Schneider
Jul 10, 2004·Cell·Derrick H LenzBonnie L Bassler
Oct 22, 2005·International Journal of Medical Microbiology : IJMM·Helge KarchMartina Bielaszewska
Jun 20, 2006·Immunology Letters·Hoon H SunwooJeong S Sim
Sep 11, 2007·Virology·Andrew M KropinskiMichael R McConnell
Feb 13, 2009·FEMS Microbiology Letters·Lynda L PerryBruce M Applegate
Apr 14, 2009·Nature Methods·Daniel G GibsonHamilton O Smith
Dec 18, 2010·Analytical and Bioanalytical Chemistry·Abby E Smartt, Steven Ripp
Jan 25, 2011·Applied and Environmental Microbiology·Minsik Kim, Sangryeol Ryu
Aug 16, 2011·Biosensors & Bioelectronics·David J YouJeong-Yeol Yoon
Nov 17, 2011·Virulence·Christian Chauret
Nov 22, 2011·Analytical and Bioanalytical Chemistry·Abby E SmarttSteven Ripp
Jun 19, 2012·Applied and Environmental Microbiology·Rong WangJoseph M Bosilevac
Jun 21, 2012·The Journal of Biological Chemistry·Ankoor Roy, Gino Cingolani
Mar 14, 2013·PLoS Genetics·William CenensAbram Aertsen
May 9, 2014·Analytical Chemistry·Seongmi KimSangryeol Ryu
Mar 15, 2016·Cell Systems·Hiroki AndoTimothy K Lu
Apr 24, 2016·Environmental Science and Pollution Research International·Si BonettaE Carraro
Sep 15, 2016·Scientific Reports·Dandan ZhangBruce Applegate
Citations
Jul 13, 2018·Annual Review of Virology·Sebastien LemireTimothy K Lu
Nov 21, 2019·Microorganisms·Shuai WeiJuhee Ahn
Oct 4, 2018·Scientific Reports·Troy C HinkleySam R Nugen
Apr 5, 2020·Sensors·Troy C HinkleySam R Nugen
Apr 21, 2019·AMB Express·Nicharee WisuthiphaetNitin Nitin
Aug 30, 2020·Viruses·Susanne MeileMatthew Dunne
May 24, 2020·Biosensors·Nasrin RazmiOmer Nur
Aug 7, 2020·Viruses·Jan PaczesnyRobert Hołyst
Jan 12, 2021·Biosensors & Bioelectronics·Wajid HussainShenqi Wang
Oct 17, 2020·Scientific Reports·Minh M NguyenStephen Erickson
Dec 22, 2019·Current Opinion in Biotechnology·Phil Huss, Srivatsan Raman
Jun 29, 2021·ACS Applied Bio Materials·Hannah S ZurierSam R Nugen
Aug 28, 2021·International Journal of Molecular Sciences·Mingxin PiaoZecheng Zuo
Aug 24, 2021·Frontiers in Microbiology·Nicharee WisuthiphaetNitin Nitin
Aug 28, 2021·Foods·Nathanyelle Soraya Martins de AquinoEduardo Cesar Tondo