Sensitive detection of viable Escherichia coli O157:H7 from foods using a luciferase-reporter phage phiV10lux

International Journal of Food Microbiology
Jinwoo KimSangryeol Ryu

Abstract

Escherichia coli O157:H7, a major foodborne pathogen, is a major public health concern associated with life-threatening diseases such as hemolytic uremic syndrome. To alleviate this burden, a sensitive and rapid system is required to detect this pathogen in various kinds of foods. Herein, we propose a phage-based pathogen detection method to replace laborious and time-consuming conventional methods. We engineered an E. coli O157:H7-specific phage phiV10 to rapidly and sensitively detect this notorious pathogen. The luxCDABE operon was introduced into the phiV10 genome and allowed the engineered phage phiV10lux to generate bioluminescence proportional to the number of viable E. coli O157:H7 cells without any substrate addition. The phage phiV10lux was able to detect at least 1CFU/ml of E. coli O157:H7 in a pure culture within 40min after 5h of pre-incubation. In artificially contaminated romaine lettuce, apple juice (pH3.51), and ground beef, the reporter phage could detect approximately 10CFU/cm(2), 13CFU/ml, and 17CFU/g of E. coli O157:H7, respectively. Taken together, the constructed reporter phage phiV10lux could be applied as a powerful tool for rapid and sensitive detection of live E. coli O157:H7 in foods.

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Citations

Jul 13, 2018·Annual Review of Virology·Sebastien LemireTimothy K Lu
Jan 12, 2021·Biosensors & Bioelectronics·Wajid HussainShenqi Wang
Oct 17, 2020·Scientific Reports·Minh M NguyenStephen Erickson
Dec 22, 2019·Current Opinion in Biotechnology·Phil Huss, Srivatsan Raman
Jun 29, 2021·ACS Applied Bio Materials·Hannah S ZurierSam R Nugen
Aug 28, 2021·International Journal of Molecular Sciences·Mingxin PiaoZecheng Zuo
Aug 24, 2021·Frontiers in Microbiology·Nicharee WisuthiphaetNitin Nitin
Aug 28, 2021·Foods·Nathanyelle Soraya Martins de AquinoEduardo Cesar Tondo

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