Sensitive determination of 4-(4-chlorophenyl)-4-hydroxypiperidine, a metabolite of haloperidol, in a rat biological sample by HPLC with fluorescence detection after pre-column derivatization using 4-fluoro-7-nitro-2,1,3-benzoxadiazole

Biomedical Chromatography : BMC
Yasuhiko HigashiYouichi Fujii

Abstract

4-(4-Chlorophenyl)-4-hydroxypiperidine (CPHP), one of the metabolites of haloperidol, is considered to exhibit brain toxicity. CPHP concentrations in plasma and tissue homogenates (each 200 microL) from rats were analyzed by HPLC fluorescence detection after pre-column derivatization with 4-fluoro-7-nitro-2,1,3-benzoxadiazole (NBD-F). After basic extraction of the samples with benzene, the derivatization with NBD-F was conducted in borate buffer (pH 8.0) at 60 degrees C for 3 min. Mexiletine was carried through the procedure as an internal standard. The regression equation for CPHP showed a good linearity in the range of 0.03-1 microg/mL with a detection limit of 0.008 microg/mL. The coefficient of variation was less than 11.6%. Plasma concentration-time courses of CPHP after intraperitoneal or per oral administration of CPHP, haloperidol or reduced haloperidol were examined, and the pharmacokinetic parameters were estimated. Additionally, CPHP levels in various tissues at 8 h after intraperitoneal administration of these compounds were compared. The method was simple and sensitive, useful for determination of CPHP in rat biological samples using as little as 200 microL of sample volume and could be applied for pharmacokinetic ...Continue Reading

References

May 28, 1992·Biochemical Pharmacology·S Y AblordeppeyW M Davis
Dec 1, 1991·The British Journal of Psychiatry : the Journal of Mental Science·K OtaniY Fukushima
Apr 1, 1981·The American Journal of Psychiatry·S H Snyder
May 1, 1993·Xenobiotica; the Fate of Foreign Compounds in Biological Systems·J W Gorrod, J Fang
Jan 1, 1996·The Journal of Neuropsychiatry and Clinical Neurosciences·I CreeseS H Snyder
Mar 23, 2002·Journal of the American Society for Mass Spectrometry·Tetsuya ArinobuOsamu Suzuki
Jul 20, 2002·Journal of Chromatography. B, Analytical Technologies in the Biomedical and Life Sciences·Tetsuya ArinobuHiroshi Seno
Feb 18, 2005·Proceedings of the National Academy of Sciences of the United States of America·Francesco FornaiThomas C Südhof

❮ Previous
Next ❯

Related Concepts

Related Feeds

Antipsychotic Drugs

Antipsychotic drugs are a class of medication primarily used to manage psychosis (including delusions, hallucinations, paranoia or disordered thought), principally in schizophrenia and bipolar disorder. Discover the latest research on antipsychotic drugs here