Sensitive determination of glutathione in biological samples by capillary electrophoresis with green (515 nm) laser-induced fluorescence detection

Journal of Chromatography. a
Júlia HodákováPetr Kubáň

Abstract

A new sensitive capillary electrophoretic method with laser-induced fluorescence (LIF) was developed for quantitation of glutathione (GSH) in biological samples. Eosin-5-maleimide was used to label the GSH molecule and the formed conjugate was separated in a 15 mM 4-(2-hydroxyethyl)-1-piperazineethanesulfonic acid electrolyte at pH 7.0 in less than 3 min. The conjugate was detected with an in-house built LIF system, utilizing an inexpensive 515 nm diode laser module. Studies were performed to optimize the derivatization (the ratio of reagent to analyte, the reaction time, pH, etc.) and separation conditions. Sensitive detection of GSH at concentrations as low as 0.18 nM was obtained. The method was applied in the analysis of biological fluids (exhaled breath condensate, saliva) and was found to be suitable for determination of GSH in these samples at trace levels below 1 nM. To the best of our knowledge, this is the first report on determination of GSH in exhaled breath condensate by capillary electrophoresis (CE).

References

Jan 1, 1992·Life Sciences·S Uhlig, A Wendel
Feb 1, 1990·Analytical Biochemistry·B J MillsC A Lang
Aug 1, 1985·Archives of Biochemistry and Biophysics·L H Lash, D P Jones
Jan 1, 1983·Annual Review of Biochemistry·A Meister, M E Anderson
Apr 29, 1983·Science·A Meister
Sep 14, 2000·Journal of Chromatography. B, Biomedical Sciences and Applications·S H KangE S Yeung
Jan 5, 2002·Spectrochimica Acta. Part A, Molecular and Biomolecular Spectroscopy·H WangH S Zhang
Nov 2, 2002·American Journal of Respiratory and Critical Care Medicine·Massimo CorradiEugenio Baraldi
Nov 27, 2002·Journal of Chromatography. B, Analytical Technologies in the Biomedical and Life Sciences·Emanuela Camera, Mauro Picardo
Apr 23, 2003·Journal of Chromatography. B, Analytical Technologies in the Biomedical and Life Sciences·K GovindarajuD H Eidelman
Dec 4, 2003·Free Radical Biology & Medicine·Daniela GiustariniRanieri Rossi
Mar 9, 2004·Electrophoresis·Ciriaco CarruAngelo Zinellu
Apr 6, 2004·Journal of Chromatography. B, Analytical Technologies in the Biomedical and Life Sciences·Angelo ZinelluLuca Deiana
Jun 10, 2004·Electrophoresis·Christophe BayleFrançois Couderc
Jul 27, 2005·Spectrochimica Acta. Part A, Molecular and Biomolecular Spectroscopy·Leyu WangFei Wang
May 13, 2006·Clinical Chemistry·Ranieri RossiDaniela Giustarini
Jun 24, 2006·Oral Diseases·D J AframianR Benoliel
Jan 5, 2007·Analytical and Bioanalytical Chemistry·Alessandro MusengaMaria Augusta Raggi
Aug 30, 2008·Journal of Chromatography. B, Analytical Technologies in the Biomedical and Life Sciences·Gordana Zunić, Slavica Spasić
Jun 30, 2009·Journal of Chromatography. B, Analytical Technologies in the Biomedical and Life Sciences·Péter MonostoriSándor Túri
Jul 22, 2009·Journal of Chromatography. B, Analytical Technologies in the Biomedical and Life Sciences·Yusuke IwasakiHiroyuki Nakazawa
Oct 28, 2009·Journal of Chromatography. B, Analytical Technologies in the Biomedical and Life Sciences·Li-Yao Zhang, Meng-Xiang Sun
Jan 9, 2014·Respiratory Research·Maria I RossoTheresa W Gauthier

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Citations

Jul 22, 2016·Journal of Chromatography. B, Analytical Technologies in the Biomedical and Life Sciences·Yao SunJiang Zheng
Nov 7, 2016·Spectrochimica Acta. Part A, Molecular and Biomolecular Spectroscopy·Yinhuan LiRuyi Zhang
Jun 16, 2017·Molecular Carcinogenesis·Renu PandeyStefano Tiziani
Aug 2, 2017·Electrophoresis·Alain Wuethrich, Joselito P Quirino
Sep 10, 2016·Electrophoresis·Júlia LačnáPetr Kubáň
Aug 13, 2017·Electrophoresis·Yuanyuan LiuChao Yan
Jul 27, 2018·Analytical and Bioanalytical Chemistry·Elaheh RahimpourAbolghasem Jouyban
May 23, 2021·Journal of Chromatography. B, Analytical Technologies in the Biomedical and Life Sciences·Manman WangQingcui Chu
Jul 13, 2021·Analytica Chimica Acta·Hai Yen TaFrancois Couderc

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