Sensitive determination of mixtures of neonicotinoid and fungicide residues in pollen and single bumblebees using a scaled down QuEChERS method for exposure assessment

Analytical and Bioanalytical Chemistry
Arthur DavidElizabeth M Hill

Abstract

To accurately estimate exposure of bees to pesticides, analytical methods are needed to enable quantification of nanogram/gram (ng/g) levels of contaminants in small samples of pollen or the individual insects. A modified QuEChERS extraction method coupled with ultra-high-performance liquid chromatography tandem mass spectrometry (UHPLC-MS/MS) analysis was tested to quantify residues of 19 commonly used neonicotinoids and fungicides and the synergist, piperonyl butoxide, in 100 mg samples of pollen and in samples of individual bumblebees (Bombus terrestris). Final recoveries ranged from 71 to 102 % for most compounds with a repeatability of below 20 % for both pollen and bumblebee extracts spiked at 5 and 40 ng/g. The method enables the detection of all compounds at sub-ng/g levels in both matrices and the method detection limits (MDL) ranged from 0.01 to 0.84 ng/g in pollen and 0.01 to 0.96 ng/g in individual bumblebees. Using this method, mixtures of neonicotinoids (thiamethoxam, clothianidin, imidacloprid and thiacloprid) and fungicides (carbendazim, spiroxamine, boscalid, tebuconazole, prochloraz, metconazole, fluoxastrobin, pyraclostrobin and trifloxystrobin) were detected in pollens of field bean, strawberry and raspberry...Continue Reading

References

Sep 7, 2007·Annual Review of Entomology·D GoulsonB Darvill
Feb 11, 2010·Journal of Chromatography. a·Steven J LehotayNatchanun Leepipatpiboon
Apr 3, 2014·Environmental Toxicology and Chemistry·Anne FairbrotherRichard Fell
Apr 11, 2014·PloS One·Francisco Sanchez-Bayo, Koichi Goka
Apr 22, 2014·The Science of the Total Environment·Konstantinos M KasiotisKyriaki Machera
Oct 24, 2014·Annual Review of Entomology·Reed M Johnson
Apr 13, 2015·Journal of Chromatography. B, Analytical Technologies in the Biomedical and Life Sciences·Malgorzata Gbylik-SikorskaAndrzej Posyniak
May 2, 2015·Environmental Science and Pollution Research International·C ChristiaK Fytianos
Oct 7, 2015·Environmental Science & Technology·Cristina BotíasDave Goulson

❮ Previous
Next ❯

Citations

May 15, 2016·Chemosphere·Claire JabotEmmanuelle Vulliet
Mar 21, 2018·Critical Reviews in Analytical Chemistry·Żaneta BargańskaJacek Namieśnik
Oct 1, 2020·Proceedings. Biological Sciences·Clara Stuligross, Neal M Williams
Oct 12, 2018·Molecules : a Journal of Synthetic Chemistry and Natural Product Chemistry·Żaneta BargańskaJacek Namieśnik
Jun 18, 2020·Molecules : a Journal of Synthetic Chemistry and Natural Product Chemistry·Maura J HallJoel R Coats
Nov 7, 2017·Environmental Science and Pollution Research International·Chiara GiorioJean-Marc Bonmatin
May 11, 2018·Analytical Sciences : the International Journal of the Japan Society for Analytical Chemistry·Yuki HirakawaShiro Miyake
Aug 17, 2020·Environmental Research·Elena ZiogaJane C Stout
Apr 14, 2021·The Science of the Total Environment·Ying ZhouJunyu Guo
Jul 22, 2018·Journal of Agricultural and Food Chemistry·Laurence Lachat, Gaétan Glauser

❮ Previous
Next ❯

Related Concepts

Related Feeds

Antifungals

An antifungal, also known as an antimycotic medication, is a pharmaceutical fungicide or fungistatic used to treat and prevent mycosis such as athlete's foot, ringworm, candidiasis, cryptococcal meningitis, and others. Discover the latest research on antifungals here.