Sensitive determinations of stable nitrogen isotopic composition of organic nitrogen through chemical conversion into N2O

Rapid Communications in Mass Spectrometry : RCM
Urumu TsunogaiF Nakagawa

Abstract

We present a method for high-sensitivity nitrogen isotopic analysis of particulate organic nitrogen (PON) in seawater and freshwater, for the purpose of determining the aquatic nitrogen fixation rate through the 15N2 tracer technique for samples that contain a low abundance of organisms. The method is composed of the traditional oxidation/reduction methods, such as the oxidation of PON to nitrate (NO3*) using persulfate, the reduction of NO3* to nitrite (NO2*) using spongy cadmium, and further reduction of NO2* to nitrous oxide (N2O) using sodium azide. Then, N2O is purged from the water and trapped cryogenically with subsequent release into a gas chromatography column to analyze the stable nitrogen isotopic composition using continuous-flow isotope ratio mass spectrometry (CF-IRMS) by simultaneously monitoring the NO+ ion currents at masses 30, 31, and 32. The nitrogen isotopic fractionation was consistent within each batch of analysis. The standard deviation of sample measurements was less than 0.3 per thousand for samples containing PON of more than 50 nmolN, and 0.5 per thousand for those of more than 20 nmolN, by subtracting the contribution of blank nitrogen, 8 +/- 2 nmol at final N2O. By using this method, we can determi...Continue Reading

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Citations

Apr 25, 2008·Rapid Communications in Mass Spectrometry : RCM·Daisuke D KomatsuUrumu Tsunogai
Aug 27, 2010·Rapid Communications in Mass Spectrometry : RCM·K KobaK Senoo
Nov 17, 2010·Rapid Communications in Mass Spectrometry : RCM·Petra LachouaniWolfgang Wanek
Mar 25, 2014·Analytical Chemistry·Dongwei LiuYuepeng Pan
Aug 24, 2018·Rapid Communications in Mass Spectrometry : RCM·Fernando Gázquez, Mark W Claire
May 12, 2018·Environmental Monitoring and Assessment·Chaopu TiXiaoyuan Yan

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