Sensitive differential detection of genetically related mycobacterial pathogens in archival material

American Journal of Clinical Pathology
J A IkonomopoulosC N Kittas

Abstract

A polymerase chain reaction (PCR) assay targeted to the immunogenic protein MPB64 gene was used to detect members of the Mycobacterium tuberculosis complex, and an outward-primed PCR (OPPCR) designed on the IS6110 element allowed differentiation between Mycobacterium bovis and Mycobacterium tuberculosis. Additionally, the amplification of IS1110 and 16S ribosomal RNA sequences combined with a dot blotting assay were able to differentially detect Mycobacterium avium, Mycobacterium intracellulare, and Mycobacterium paratuberculosis. The validity of the experimental procedure was tested on reference material and formalin-fixed paraffin-embedded samples from patients with tuberculosis, sarcoidosis, or Crohn disease. We demonstrated mycobacterial DNA in 59 of 75 cases with histologic lesions typical of tuberculosis; we detected M tuberculosis and M paratuberculosis in 6 of 25 sarcoidosis cases and in 7 of 20 Crohn disease specimens, respectively. The proposed diagnostic procedure is directly applicable to archival material and allows differentiation of genetically related mycobacterial pathogens in more detail than other molecular methods. It provides a tool for the diagnostic study of tuberculosis, sarcoidosis, and Crohn disease.

References

Jul 1, 1992·Gut·J D SandersonJ Hermon-Taylor
Mar 1, 1991·The Journal of Histochemistry and Cytochemistry : Official Journal of the Histochemistry Society·J Ben-EzraA Wu
Jan 1, 1991·Gut·K TanakaS Tabaqchali
Oct 1, 1990·International Journal of Systematic Bacteriology·T RogallE C Böttger
Feb 1, 1995·Veterinary Microbiology·B J WardsG W de Lisle
Jul 1, 1994·Journal of Clinical Microbiology·B A Forbes, K E Hicks
Jun 1, 1994·Molecular Microbiology·M Hernandez PerezJ W Dale
Mar 1, 1994·Journal of Clinical Microbiology·Z LiM J Brennan
May 1, 1993·Journal of Clinical Microbiology·D ThierryJ L Guesdon
Jun 1, 1996·Journal of Clinical Microbiology·J BennedsenE G Wilkins
Sep 1, 1996·Journal of Clinical Microbiology·F A el-ZaatariD Y Graham
Oct 1, 1996·Journal of Clinical Microbiology·H NeimarkS Carleton
Jan 1, 1997·American Journal of Respiratory and Critical Care Medicine·W J BurmanM D Cave
Jul 1, 1997·Human Pathology·H H PopperE Winter
Sep 1, 1997·Nucleic Acids Research·S F AltschulD J Lipman

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Citations

Jun 23, 2009·Journal of Microbiological Methods·Emmanouil LiandrisJohn Ikonomopoulos
Aug 24, 2007·The Lancet Infectious Diseases·Martin FellerMatthias Egger
Jan 21, 2016·International Journal of Mycobacteriology·Esmaeil MortazPeter J Barnes
Dec 17, 2008·Revista Do Instituto De Medicina Tropical De São Paulo·Denise BarcelosSylvia Cardoso Leão
Apr 1, 2008·Expert Review of Respiratory Medicine·Surinder K Jindal
Apr 26, 2006·Respiration; International Review of Thoracic Diseases·Tiberiu ShulimzonIssahar Ben-Dov
Apr 19, 2006·Journal of the Formosan Medical Association = Taiwan Yi Zhi·Chi-Yuan TzenChin-Yuan Tzen

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