Sensitive enzymatic quantification of 5-hydroxymethylcytosine in genomic DNA

Nucleic Acids Research
Aleksandra SzwagierczakHeinrich Leonhardt

Abstract

The recent discovery of genomic 5-hydroxymethylcytosine (hmC) and mutations affecting the respective Tet hydroxylases in leukemia raises fundamental questions about this epigenetic modification. We present a sensitive method for fast quantification of genomic hmC based on specific transfer of radiolabeled glucose to hmC by a purified glucosyltransferase. We determined hmC levels in various adult tissues and differentiating embryonic stem cells and show a correlation with differential expression of tet genes.

References

Feb 1, 1972·The Biochemical Journal·N W PennR Yura
May 1, 1971·Virology·C P Georgopoulos, H R Revel
Jul 11, 2006·Genes to Cells : Devoted to Molecular & Cellular Mechanisms·Akiko TsumuraMasaki Okano
Apr 18, 2009·Science·Skirmantas Kriaucionis, Nathaniel Heintz
Jun 2, 2009·Nature Genetics·Saskia M C LangemeijerJoop H Jansen
Jun 25, 2009·Chemistry & Biology·Christoph Loenarz, Christopher J Schofield
Jul 1, 2009·Journal of Cellular Biochemistry·Andrea RottachFabio Spada

Citations

Nov 1, 2012·Chromosome Research : an International Journal on the Molecular, Supramolecular and Evolutionary Aspects of Chromosome Biology·Musashi KubiuraMasako Tada
Sep 3, 2011·Cell Stem Cell·Luisa CimminoIannis Aifantis
Apr 15, 2011·Nature·Kristine WilliamsKristian Helin
May 10, 2011·Nature·William A PastorAnjana Rao
Nov 10, 2012·Nature Biotechnology·Chun-Xiao SongChuan He
Jul 12, 2012·Neuropsychopharmacology : Official Publication of the American College of Neuropsychopharmacology·Lisa D MooreGuoping Fan
Jan 28, 2012·Nature Protocols·Adam B RobertsonArne Klungland
Dec 1, 2012·Nature Protocols·Miao YuChuan He
Jul 31, 2012·Chemical Society Reviews·Edita KriukienėSaulius Klimašauskas
Apr 30, 2013·Briefings in Functional Genomics·Benjamin Delatte, François Fuks
Jul 28, 2011·DNA Research : an International Journal for Rapid Publication of Reports on Genes and Genomes·Lena ScheubertGeorg Fuellen
May 15, 2013·Human Molecular Genetics·Fengli WangTie-Shan Tang
Jan 17, 2012·Journal of Biochemistry·Yu-ichi Tsukada
Mar 8, 2011·Nucleic Acids Research·Aleksandra SzwagierczakFabio Spada
Mar 8, 2011·Nucleic Acids Research·Seung-Gi JinGerd P Pfeifer
Dec 14, 2011·Genes & Development·Hao Wu, Yi Zhang
Jul 26, 2011·Genome Research·Diane I SchroederJanine LaSalle
Nov 23, 2011·Genome Research·Colm E NestorRichard R Meehan
Jun 19, 2013·BMC Genomics·Béatrice de MonteraClaude Robert
Aug 21, 2013·Genome Biology·Maxim IvanovMagnus Ingelman-Sundberg
May 10, 2012·Development·Li Tan, Yujiang Geno Shi
Jul 7, 2011·PloS One·Carina FrauerHeinrich Leonhardt
Jun 5, 2012·Yi chuan = Hereditas·Yan-Xia ZhangShun-Ying Yu
Jul 21, 2011·Journal of Nucleic Acids·Weiwei Li, Min Liu
Dec 1, 2011·Epigenomics·Chun-Xiao Song, Chuan He
May 27, 2014·Current Environmental Health Reports·T DaoWy Tang
Feb 11, 2014·Biomedicine & Pharmacotherapy = Biomédecine & Pharmacothérapie·Bartosz Adam FryczPaweł P Jagodziński
May 13, 2014·Cell and Tissue Research·Gerd P PfeiferSeung-Gi Jin
Apr 24, 2014·Proceedings of the National Academy of Sciences of the United States of America·Xiang LiTimothy W Bredy
Aug 1, 2014·Journal of the American Society for Mass Spectrometry·Shuo LiuYinsheng Wang
Jul 30, 2014·Analytical Chemistry·Tamar ShahalYuval Ebenstein
Sep 15, 2012·Annual Review of Genetics·Don-Marc FranchiniSvend K Petersen-Mahrt
Aug 28, 2014·Nature Reviews. Genetics·Nongluk PlongthongkumKun Zhang
Feb 28, 2013·Chemical Reviews·Natalia TretyakovaSrikanth Kotapati
May 17, 2013·Photochemical & Photobiological Sciences : Official Journal of the European Photochemistry Association and the European Society for Photobiology·Sang-in KimGerd P Pfeifer
Jun 23, 2011·Chemical Communications : Chem Comm·Akiko NomuraAkimitsu Okamoto
Sep 16, 2011·Chemical Communications : Chem Comm·Akimitsu OkamotoShuji Ikeda
May 24, 2013·Nature Reviews. Molecular Cell Biology·William A PastorAnjana Rao
Feb 9, 2016·Cell Reports·Georgia Rose KaferPeter Mark Carlton
Dec 10, 2014·International Journal of Molecular Sciences·Huan MengLiu Cao
Jan 27, 2015·ACS Synthetic Biology·David J SukovichJ Christopher Anderson
Jan 26, 2016·Neuroscience Letters·Yasaman AlaghbandMarcelo A Wood
Dec 4, 2015·International Journal of Molecular Sciences·Hiromichi AkahoriYoshinori Muto
Dec 20, 2011·Trends in Biochemical Sciences·Danny C Leung, Matthew C Lorincz
Feb 4, 2016·Stem Cells International·Alexandra J HarveyDavid K Gardner
Aug 16, 2015·Critical Reviews in Oncology/hematology·Agnieszka Anna Rawłuszko-WieczorekPaweł P Jagodziński
Jun 10, 2014·Scientific Reports·Toshihiro ShibutaniIsao Kuraoka
Jan 27, 2011·Journal of Cellular Biochemistry·Weihua QinFabio Spada
Jan 24, 2013·Cancer Medicine·Ekaterina Olkhov-Mitsel, Bharati Bapat
Dec 14, 2011·EMBO Reports·Kristine WilliamsKristian Helin
May 21, 2011·Journal of Cellular Biochemistry·Garwin PichlerHeinrich Leonhardt
Jul 18, 2015·Epigenetics : Official Journal of the DNA Methylation Society·Christian GrosserBernhard Horsthemke
Jul 30, 2014·Alcoholism, Clinical and Experimental Research·Stephanie A TammenSang-Woon Choi
Jan 22, 2015·European Journal of Clinical Investigation·Dirk Schübeler
Feb 25, 2015·Development, Growth & Differentiation·Ke TangNaihe Jing
Dec 17, 2014·Immunological Reviews·Myunggon KoAnjana Rao
Sep 18, 2015·Frontiers in Cellular Neuroscience·Paola TogniniTommaso Pizzorusso
Jun 5, 2015·Human Molecular Genetics·Sruti ChandraMelanie Ehrlich
Jul 28, 2012·Current Opinion in Neurobiology·Volkan CoskunYi E Sun
Sep 18, 2012·Biochimica Et Biophysica Acta·Roland Faigle, Hongjun Song
May 20, 2015·Bioconjugate Chemistry·Saulius Serva, Arūnas Lagunavičius
Jun 21, 2015·Brain Research·Sarah MoyonPatrizia Casaccia
Mar 16, 2012·Epigenetics : Official Journal of the DNA Methylation Society·Rimple D AlmeidaAlexey Ruzov
Mar 8, 2012·Epigenetics : Official Journal of the DNA Methylation Society·Rimple D AlmeidaAlexey Ruzov
Sep 21, 2013·Mutation Research. Genetic Toxicology and Environmental Mutagenesis·Jean Cadet, J Richard Wagner
Feb 8, 2011·Cell Stem Cell·Jörn Walter
Jul 6, 2014·Blood·Leonie I KroezeEORTC Leukemia Group and GIMEMA
Jan 7, 2014·Neurobiology of Aging·Natacha CoppietersMike Dragunow
Feb 26, 2013·Cell·Cornelia G SpruijtMichiel Vermeulen
Sep 27, 2014·The Science of the Total Environment·Jie ZhangHeqing Shen
Mar 17, 2015·Nature Neuroscience·Jian FengEric J Nestler
Jun 19, 2013·Cell Stem Cell·Run-Rui ZhangGuo-Liang Xu
Jun 26, 2012·Journal of Theoretical Biology·Andrew P McGovernTimothy J T Chevassut
Sep 3, 2014·Genomics·Gabriella Ficz, John G Gribben
Feb 26, 2015·Journal of Immunoassay & Immunochemistry·Laura Georgiana NeculaCarmen C Diaconu
May 26, 2012·Biochemical and Biophysical Research Communications·Ning HuangHao Wang
Jun 28, 2011·Biochemical and Biophysical Research Communications·Julia RobertsonArne Klungland
Jan 22, 2013·Biophysical Journal·Philip M D SeverinHermann E Gaub
Sep 18, 2012·Cell·Christine G LianYujiang G Shi
Jun 10, 2011·Blood·William VainchenkerOlivier A Bernard
Jun 21, 2014·Nucleic Acids Research·Udo MüllerHeinrich Leonhardt
Nov 29, 2014·Epigenomics·Euan J RodgerIan M Morison
Dec 3, 2014·Progress in Neurobiology·Qingyi Ma, Lubo Zhang
May 7, 2016·Nature Reviews. Neuroscience·Annie Vogel Ciernia, Janine LaSalle
May 18, 2016·ELife·Marketa TomkovaBenjamin Schuster-Boeckler
Jun 28, 2016·General and Comparative Endocrinology·Daniela VenegasHoracio Merchant-Larios
Sep 23, 2016·Biochemistry·Crystal M Vander ZandenP Shing Ho
Mar 24, 2017·BioEssays : News and Reviews in Molecular, Cellular and Developmental Biology·Ying QingJiajie Diao
Apr 26, 2017·RNA Biology·Franziska R Traube, Thomas Carell
Dec 8, 2016·Nucleic Acids Research·Anne K LudwigM Cristina Cardoso
May 16, 2017·Genes·Maria Fasolino, Zhaolan Zhou
Mar 28, 2017·Genome Research·Harmen J G van de WerkenBoris Joffe
Feb 17, 2018·Frontiers in Neuroscience·Stefan H Stricker, Magdalena Götz
Jun 19, 2018·Nucleic Acids Research·Pascal GiehrJörn Walter
Jul 2, 2011·Angewandte Chemie·Toni PfaffenederThomas Carell
Jun 21, 2011·Angewandte Chemie·Martin MünzelThomas Carell
Aug 23, 2018·Gynecological Endocrinology : the Official Journal of the International Society of Gynecological Endocrinology·Małgorzata SzczepańskaPaweł P Jagodziński
Dec 23, 2018·Journal of Separation Science·James AsensoDao-Quan Tang
Jan 28, 2018·Scientific Reports·Eloïse A KremerIsabelle M Mansuy
Jul 3, 2019·Epigenetics : Official Journal of the DNA Methylation Society·Nikhil JainYuval Ebenstein
Oct 10, 2013·Chembiochem : a European Journal of Chemical Biology·Nan DaiIvan R Corrêa
Mar 23, 2017·EMBO Reports·Dewei JiangJiali Li
May 16, 2014·The EMBO Journal·Benjamin DelatteFrançois Fuks
Sep 12, 2019·Epigenetics : Official Journal of the DNA Methylation Society·Nicholas D JohnsonKaren N Conneely
Jan 13, 2018·PloS One·Fei WangRuth Steward
Oct 25, 2019·Cellular and Molecular Life Sciences : CMLS·Mafalda SantiagoC Joana Marques
Dec 14, 2019·Aging Cell·Luigi FerrucciRafael de Cabo
Feb 5, 2020·Genes, Chromosomes & Cancer·Catarina SalgadoRemco van Doorn
Jan 11, 2020·AIMS Genetics·Asaad M Mahmood, Jim M Dunwell
Nov 5, 2017·Oncotarget·Siqi FuQianjin Lu
Apr 3, 2016·Genes & Development·Kasper Dindler Rasmussen, Kristian Helin
Mar 24, 2017·Human Molecular Genetics·Martha SmetsHeinrich Leonhardt
Mar 15, 2019·Nature Reviews. Genetics·Özgen DenizMiguel R Branco
Feb 24, 2011·Angewandte Chemie·Zita LiutkevičiūtėSaulius Klimašauskas
Jun 10, 2011·Chembiochem : a European Journal of Chemical Biology·Chun-Xiao SongChuan He
Apr 13, 2011·Angewandte Chemie·Claudia Höbartner
Nov 9, 2014·G3 : Genes - Genomes - Genetics·Robert M ErdmannMary Gehring
Jan 9, 2015·The Journal of Biological Chemistry·Christina BauerHeinrich Leonhardt
May 17, 2018·Brain Plasticity·Emily M Jobe, Xinyu Zhao
Mar 21, 2018·Frontiers in Cell and Developmental Biology·Philippa MelamedLilach Pnueli
Jan 5, 2019·Epigenetics & Chromatin·Dimitrios CakourosStan Gronthos
May 9, 2020·Journal of Cerebral Blood Flow and Metabolism : Official Journal of the International Society of Cerebral Blood Flow and Metabolism·Kahlilia C Morris-BlancoRaghu Vemuganti
Nov 10, 2013·Cancers·Christopher J MarianiLucy A Godley
May 23, 2017·Cell Discovery·Miriam LandforsArne Klungland
Apr 30, 2017·Experimental & Molecular Medicine·Jungeun AnMyunggon Ko
Jul 18, 2018·Cells·Elkin Navarro QuirozGustavo Aroca Martinez
Jun 19, 2019·Biochemical Society Transactions·Samuel E Ross, Ozren Bogdanovic
Jan 13, 2015·Journal of Cancer Prevention·Stephanie A TammenSang-Woon Choi
Aug 4, 2018·Analytical Cellular Pathology (Amsterdam)·Anubhav Chakraborty, Pragasam Viswanathan
Jan 30, 2019·Annual Review of Medicine·Martine W Tremblay, Yong-Hui Jiang
Oct 12, 2020·Methods : a Companion to Methods in Enzymology·Shizhao Li, Trygve O Tollefsbol
Apr 3, 2020·Developmental Biology·Yasuhiro KyonoRobert J Denver
Mar 9, 2021·Frontiers in Cell and Developmental Biology·Ian C MacArthur, Meelad M Dawlaty
Oct 16, 2020·FASEB Journal : Official Publication of the Federation of American Societies for Experimental Biology·Ying ZhangJiali Li
Jan 9, 2015·The Journal of Experimental Biology·Gabriella Ficz
May 8, 2021·Epigenetics : Official Journal of the DNA Methylation Society·Maud De DieuleveultRachel Deplus

Methods Mentioned

BETA
deamination
glucosylation
gel filtration
electrophoresis
PCR
ion exchange chromatography

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Hereditary Sensory Autonomic Neuropathy

Hereditary Sensory Autonomic Neuropathies are a group of inherited neurodegenerative disorders characterized clinically by loss of sensation and autonomic dysfunction. Here is the latest research on these neuropathies.

Spatio-Temporal Regulation of DNA Repair

DNA repair is a complex process regulated by several different classes of enzymes, including ligases, endonucleases, and polymerases. This feed focuses on the spatial and temporal regulation that accompanies DNA damage signaling and repair enzymes and processes.

Glut1 Deficiency

Glut1 deficiency, an autosomal dominant, genetic metabolic disorder associated with a deficiency of GLUT1, the protein that transports glucose across the blood brain barrier, is characterized by mental and motor developmental delays and infantile seizures. Follow the latest research on Glut1 deficiency with this feed.

Separation Anxiety

Separation anxiety is a type of anxiety disorder that involves excessive distress and anxiety with separation. This may include separation from places or people to which they have a strong emotional connection with. It often affects children more than adults. Here is the latest research on separation anxiety.

KIF1A Associated Neurological Disorder

KIF1A associated neurological disorder (KAND) is a rare neurodegenerative condition caused by mutations in the KIF1A gene. KAND may present with a wide range and severity of symptoms including stiff or weak leg muscles, low muscle tone, a lack of muscle coordination and balance, and intellectual disability. Find the latest research on KAND here.

Regulation of Vocal-Motor Plasticity

Dopaminergic projections to the basal ganglia and nucleus accumbens shape the learning and plasticity of motivated behaviors across species including the regulation of vocal-motor plasticity and performance in songbirds. Discover the latest research on the regulation of vocal-motor plasticity here.