Sensitive enzyme-biosensor based on screen-printed electrodes for Ochratoxin A

Biosensors & Bioelectronics
M Asunción Alonso-LomilloM J Arcos-Martínez

Abstract

Horseradish peroxidase has been successfully immobilized in a polypyrrole matrix onto disposable screen-printed carbon electrodes for the selective detection of Ochratoxin A. The chronoamperometric determination of this mycotoxin has been optimized by experimental design methodology, which implied the join evaluation of pH of the buffer solution, applied potential and concentration of H(2)O(2). The slopes of the calibration curves built under the optimum conditions of the experimental variables have been used to estimate the reproducibility and the repeatability of the developed biosensor for Ochratoxin A. Relative standard deviation values of 1.9% (n=5 and alpha=0.05) and 7.1% (n=4 and alpha=0.05) were obtained for reproducibility and repeatability, respectively. The capability of detection for this method was 0.1 ng mL(-1) (alpha=0.05 and beta<0.05). The viability of the developed biosensor in the determination of Ochratoxin A in spiked beer and in roasted coffee has been shown, yielding average recoveries of 103% and 99%, in that order, with relative standard deviation values less than 5% (n=3 and alpha=0.05) in both cases.

References

Sep 18, 2001·Chemical Research in Toxicology·M W CalcuttR A Manderville
Jun 6, 2003·Biosensors & Bioelectronics·M A Alonso LomilloM J Arcos Martinez
Oct 19, 2007·Journal of Agricultural and Food Chemistry·N E AhmedKh M Naguib
Nov 24, 2007·Biosensors & Bioelectronics·Beatriz Prieto-SimónThierry Noguer
Nov 18, 2008·Biosensors & Bioelectronics·Abd-Elgawad RadiJean-Louis Marty
Dec 9, 2008·Bioelectrochemistry·M Asunción Alonso-LomilloM Julia Arcos-Martínez
Dec 27, 2008·Analytica Chimica Acta·Nicholas W TurnerSergey A Piletsky

❮ Previous
Next ❯

Citations

Jan 27, 2012·Analytical and Bioanalytical Chemistry·T F McGrathT L Fodey
Jan 12, 2012·The Analyst·Mònica CampàsBeatriz Prieto-Simón
Sep 1, 2010·Toxins·Ingrid BazinMiguel Lopez-Ferber
May 12, 2012·Analytical and Bioanalytical Chemistry·Annalisa De GirolamoAngelo Visconti
Mar 19, 2016·Journal of Hazardous Materials·Varvara PagkaliSotirios E Kakabakos
Nov 29, 2015·Analytica Chimica Acta·Nicholas W TurnerSergey A Piletsky
Feb 8, 2011·Analytica Chimica Acta·M Asunción Alonso-LomilloM Julia Arcos-Martínez
Jun 8, 2013·Biosensors & Bioelectronics·Juan C VidalJuan R Castillo
Jul 22, 2014·Biosensors & Bioelectronics·Marta Sánchez-Paniagua LópezBeatriz López-Ruiz
Apr 20, 2013·Talanta·Laura Asturias-ArribasM Julia Arcos-Martínez
Aug 17, 2014·Talanta·Laura Asturias-ArribasM Julia Arcos-Martínez
Nov 27, 2010·Chemical Communications : Chem Comm·Junfei Tian, Wei Shen
Nov 6, 2013·Critical Reviews in Food Science and Nutrition·Jinyi YangDharini Sivakumar
Nov 24, 2018·Analytical and Bioanalytical Chemistry·İrem Okman KoçoğluEsma Kılıç
Aug 29, 2018·Annual Review of Phytopathology·Wade Elmer, Jason C White
Apr 8, 2020·Biosensors·Beatriz Pérez-FernándezAlfredo de la Escosura- Muñiz
Sep 3, 2014·Biotechnology, Biotechnological Equipment·Mohammad A KhiyamiKamel A Abd-Elsalam
Jan 27, 2021·Analytical Chemistry·Sara Tortorella, Stefano Cinti

❮ Previous
Next ❯

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.

© 2022 Meta ULC. All rights reserved