Sensitive immunoassays based on a monoclonal antibody for detection of marbofloxacin in milk

Journal of Dairy Science
Meixia LuoJuan Peng

Abstract

Marbofloxacin (MBF) is a key class of synthetic fluoroquinolone antibiotic that is commonly used as a veterinary drug. However, excess residue of MBF in animal-derived food samples, such as milk, is harmful to consumers. In this study, 4 mAb against MBF, namely, M4E3, M7A6, M3C7, and M5C6, were produced. Indirect competitive (ic) ELISA revealed that the M4E3 exhibited the highest sensitivity with a half-maximal inhibitory concentration (IC50) of 0.07 ng/mL and a limit of detection of 0.01 ng/mL for detection of MBF. The results of the cross-reactivity experiment revealed that the M4E3 could detect lomefloxacin, ofloxacin, fleroxacin, pefloxacin, danofloxacin, and enrofloxacin sensitively with IC50 of 0.02, 0.07, 0.18, 0.27, 0.30, and 0.32 ng/mL, respectively. Meanwhile, a cross-reactivity experiment showed that the M4E3 had a crossover rate of more than 20% with these fluoroquinolone analogs. A weak crossover rate below 1.11% was observed with 14 other fluoroquinolone analogs. The recovery rate of MBF in milk ranged from 72.28% to 129.19%, which showed that the developed indirect competitive ELISA was effective in detecting MBF in milk. Furthermore, a visual colloidal gold-based immunochromatographic assay was developed for det...Continue Reading

References

Jun 16, 2009·Journal of Agricultural and Food Chemistry·Wei ShengTing Xu
Apr 2, 2015·Environmental Science & Technology·Hexing WangQingwu Jiang
Feb 18, 2016·Environment International·Hexing WangQingwu Jiang
Apr 4, 2017·Journal of Agricultural and Food Chemistry·Marina RuschGerd Hamscher
Jun 24, 2019·Tropical Animal Health and Production·Feray AltanKamil Uney
Dec 6, 2019·MedChemComm·Thu D M PhamMark A T Blaskovich

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Citations

Jun 3, 2021·Comprehensive Reviews in Food Science and Food Safety·Yawen RongQuansheng Chen

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