Sensitive measurement of vinorelbine in dog plasma by liquid chromatography-electrospray ionization tandem mass spectrometry utilizing transitions from double-charged precursor ions

Biomedical Chromatography : BMC
Makoto Niwa, Takashi Kawashiro

Abstract

A sensitive high-performance liquid chromatography/tandem mass spectrometry (LC/MS/MS) method for measuring vinorelbine was developed. A 100 µL aliquot of plasma was spiked with deuterium-labeled internal standard and subjected to solid-phase extraction using an Oasis HLB μ-elution plate. Two microliters of the extracted samples was directly injected into LC/MS/MS. Chromatographic separation was achieved on a Capcell Pak C18 UG column (2 × 75 mm) with a gradient elution of methanol (mobile phase B) against 0.05% formic acid in aqueous 10 mm ammonium formate (mobile phase A). The LC flow rate was set to 0.28 mL/min and the gradient (solvent B concentration) was processed from 40 to 90%. In mass spectrometric detection, observation of the reaction from a double-charged precursor ion [M + 2H](2+) (m/z 390) to product ion m/z 122 provided very high sensitivity. The method was validated with a lower limit of detection of 0.2 ng/mL with 0.1 mL of plasma, and the method was used to determine the plasma pharmacokinetics of vinorelbine in dogs.

References

Jan 17, 1992·Journal of Chromatography·O van TellingenW J Nooyen
May 4, 2001·Journal of Chromatography. B, Biomedical Sciences and Applications·S RagotP Marquet
Jul 8, 2005·Cancer Chemotherapy and Pharmacology·Richard M LushDaniel M Sullivan
Apr 6, 2007·The AAPS Journal·Surendra Bansal, Anthony DeStefano
Jun 15, 2007·Cancer Chemotherapy and Pharmacology·J BonneterreI Douville
May 27, 2008·Journal of Chromatography. B, Analytical Technologies in the Biomedical and Life Sciences·Carola W N DamenJos H Beijnen
Apr 9, 2009·Biomedical Chromatography : BMC·Tiefeng ChengChangxiao Liu
Jan 14, 2010·Rapid Communications in Mass Spectrometry : RCM·Erik AllardPer Johan Ragnar Sjöberg

❮ Previous
Next ❯

Citations

Jun 1, 2017·The Analyst·Nicolas GuichardSandrine Fleury-Souverain

❮ Previous
Next ❯

Related Concepts

Related Feeds

Anxiety Disorders

Discover the latest research on anxiety disorders including agoraphobia, panic disorder, obsessive-compulsive disorder, and post-traumatic stress disorder here.