Sensitive response of cultured vascular smooth-muscle cells to cadmium cytotoxicity: comparison with cultured vascular endothelial cells and kidney epithelial LLC-PK1 cells

Toxicology Letters
T KajiH Kozuka

Abstract

Response to cadmium cytotoxicity of cultured bovine aortic smooth-muscle cells was compared with that of cultured bovine aortic endothelial cells and porcine kidney epithelial LLC-PK1 cells. The cell damage was evaluated by morphology and the lactate dehydrogenase leakage assay. It was found that vascular smooth-muscle cells are markedly sensitive to cadmium cytotoxicity. The accumulation of intracellular cadmium was much higher but that of metallothionein was much less in vascular smooth-muscle cells than in LLC-PK1 cells; vascular endothelial cells were in between vascular smooth-muscle cells and LLC-PK1 cells. The content of reduced glutathione was slightly increased by cadmium in all three cell types. The present data suggest that a much lower inducibility of metallothionein with a high accumulation of intracellular cadmium in vascular smooth-muscle cells resulted in a marked sensitivity of the cells to cadmium cytotoxicity. Vascular smooth-muscle cells may be one of the critical target of cadmium toxicity.

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Citations

Sep 6, 2005·Cell Biology and Toxicology·B L'AzouL Bordenave
Jul 10, 2009·Biological Trace Element Research·Hassan Imran AfridiMohammad Balal Arain
Sep 22, 2001·Toxicology in Vitro : an International Journal Published in Association with BIBRA·I OlabarrietaM P Cajaraville
Jul 7, 2007·Journal of Toxicology and Environmental Health. Part a·Céline Ohayon-CourtèsBéatrice L'Azou
Mar 31, 2004·Yakugaku zasshi : Journal of the Pharmaceutical Society of Japan·Toshiyuki Kaji
Mar 14, 2002·Biochemical and Biophysical Research Communications·Zhuoxiao Cao, Yunbo Li
Jun 28, 2006·Biometals : an International Journal on the Role of Metal Ions in Biology, Biochemistry, and Medicine·Vassilios TzotzesHaris Carageorgiou
Nov 26, 2014·Archives of Toxicology·Yasuyuki FujiwaraToshiyuki Kaji
Jan 31, 2016·Journal of Biological Inorganic Chemistry : JBIC : a Publication of the Society of Biological Inorganic Chemistry·Tomoya FujieToshiyuki Kaji
Oct 27, 2004·Chemico-biological Interactions·Pei-Ming YangLih-Yuan Lin
Aug 27, 2005·Human & Experimental Toxicology·A Skoczynska, H Martynowicz
May 23, 2001·Arteriosclerosis, Thrombosis, and Vascular Biology·S Abu-HayyehJ T Powell
Jun 15, 2007·Pathophysiology of Haemostasis and Thrombosis·Mehtap Koçak, Ethem Akçil
Mar 11, 2016·The Journal of Toxicological Sciences·Tomoya FujieToshiyuki Kaji

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