Oct 26, 1981

Sensitive solid phase enzyme immunoassay for human IgA, secretory IgA, and secretory component

Clinica Chimica Acta; International Journal of Clinical Chemistry
Y IshiguroT Ito

Abstract

Highly sensitive solid-phase immunoassay systems for human immunoglobulin A (IgA), secretory component (SC), secretory immunoglobulin A (SIgA) were developed by use of antisera against the alpha-chain of IgA and SC, and beta-D-galactosidase from Escherichia coli as label. IgA and SC wee assayed with the respective solid-phase (silicone rubber)-immobilized F(ab')2 antibody fragments and the corresponding antibody Fab'-beta-D-galactosidase complex. More than 1 and 0.4 fmol (or 0.16 and 0.03 ng) of IgA, and SC, respectively, were determined, but the assay system for IgA and that for SC cross-reacted with SIgA about 90% and 2%, respectively. SIgA was specifically determined in an assay using silicone rubber with immobilized (anti-alpha-chain)F(ab')2 fragments and (anti-SC) Fab'-beta-D-galactosidase complex with a minimum detectable sensitivity of 2.5 fmol or 1 ng. IgA and SC values could be corrected by subtracting the amounts of cross-reacting SIgA in the same samples. Small amounts of SIgA, SC, and IgA in saliva, sweat, urine, and feces could be determined by the present method.

Mentioned in this Paper

Urine
Silicone Elastomers
Siga
Sweat
Specimen Type - Sweat
Saliva - SpecimenType
Immune Sera
Fab Immunoglobulins
Immunoglobulin A, Human
FCGRT gene

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