Sensitivities of antigen detection and PCR assays greatly increased compared to that of the standard culture method for screening for group B streptococcus carriage in pregnant women

Journal of Clinical Microbiology
Fabien RalluCéline Laferrière

Abstract

Group B streptococcus (GBS) is a major cause of serious infections in neonates. The 2002 revised guidelines of the Centers for Disease Control and Prevention (CDC) for the prevention of perinatal GBS disease recommend that all pregnant women be screened for GBS carriage at between 35 and 37 weeks of gestation and that intrapartum antibiotic prophylaxis be given to carriers. We studied the performances of four different GBS detection assays in the context of antenatal screening. Between May and August 2004, the 605 vaginorectal swab specimens received at our bacteriology laboratory for GBS antenatal detection were tested by the four assays. The standard culture method was done according to the CDC recommendations. The three experimental assays performed with the growth from the selective enrichment (LIM) broth (Todd-Hewitt broth with 15 mug/ml nalidixic acid and 10 mug/ml colistin) after overnight incubation were a GBS antigen detection assay (PathoDx) and two PCR assays (for cfb and scpB). The most accurate assay was the scpB PCR (sensitivity, 99.6%; specificity, 100%), followed by the cfb PCR (sensitivity, 75.3%; specificity, 100%), GBS antigen detection (sensitivity, 57.3%; specificity, 99.5%), and standard culture (sensitivi...Continue Reading

References

Mar 23, 1979·JAMA : the Journal of the American Medical Association·M D YowS E Gardner
Nov 25, 1988·Nucleic Acids Research·F Corpet
Jun 1, 1982·The Journal of Infectious Diseases·H C DillonB M Gray
Jan 6, 2000·The New England Journal of Medicine·S J SchragA Schuchat
Jul 20, 2000·The New England Journal of Medicine·M G BergeronW D Fraser
Oct 20, 2001·American Journal of Obstetrics and Gynecology·K T ChenE Lieberman
Jul 26, 2002·The New England Journal of Medicine·Stephanie J SchragUNKNOWN Active Bacterial Core Surveillance Team
Oct 29, 2002·Seminars in Neonatology : SN·William E Benitz
May 30, 2003·Journal of Perinatology : Official Journal of the California Perinatal Association·Nelangi M PintoRoger G Faix
Jun 13, 2003·Lancet·Suzanne LuckAlison Bedford Russell
Jul 20, 2004·European Journal of Clinical Microbiology & Infectious Diseases : Official Publication of the European Society of Clinical Microbiology·F J Picard, M G Bergeron
Oct 16, 2004·Clinical Infectious Diseases : an Official Publication of the Infectious Diseases Society of America·H Dele DaviesJeanne A Jordan
Oct 16, 2004·Clinical Infectious Diseases : an Official Publication of the Infectious Diseases Society of America·Stephanie J Schrag
Nov 2, 2004·Obstetrics and Gynecology·Ronald S GibbsAnne Schuchat
Feb 8, 2005·Journal of Clinical Microbiology·Manuel Rosa-FraileCarmen Liébana-Martos

❮ Previous
Next ❯

Citations

Nov 22, 2007·European Journal of Clinical Microbiology & Infectious Diseases : Official Publication of the European Society of Clinical Microbiology·A AdlerA E Moses
Jul 5, 2013·Clinical and Vaccine Immunology : CVI·Hidehito MatsuiHideaki Hanaki
Sep 10, 2013·Journal of Laboratory Physicians·Kavitha P KonikkaraB Bharati
Aug 16, 2008·Journal of Perinatology : Official Journal of the California Perinatal Association·L S PulverC L Byington
Jan 25, 2016·Journal of the Chinese Medical Association : JCMA·Shahrokh BidganiMansour Amin
Apr 7, 2012·Diagnostic Microbiology and Infectious Disease·John SchwartzBobby L Boyanton
Jan 5, 2010·Taiwanese Journal of Obstetrics & Gynecology·Horng-Jyh Tsai
Jan 1, 2009·Journal of Infection and Public Health·Sarah Ahmed Abd El-Kawy ShabayekAbouzeid M H Abouzeid
May 19, 2007·Diagnostic Microbiology and Infectious Disease·Jennifer S Goodrich, Melissa B Miller
Jan 16, 2013·The Australian & New Zealand Journal of Obstetrics & Gynaecology·Ibrahim A Abdelazim
Mar 1, 2014·The Australian & New Zealand Journal of Obstetrics & Gynaecology·Warren S W ChanGwendolyn L Gilbert
Jan 19, 2008·American Journal of Obstetrics and Gynecology·John W Larsen, John L Sever
Sep 24, 2010·Clinical Microbiology and Infection : the Official Publication of the European Society of Clinical Microbiology and Infectious Diseases·B Martinez de TejadaJ Schrenzel
Oct 8, 2014·Journal of Infection and Chemotherapy : Official Journal of the Japan Society of Chemotherapy·Miyuki MorozumiKimiko Ubukata
Apr 21, 2012·Gynecologic and Obstetric Investigation·Yen-Ying MaHao Lin
Nov 29, 2017·Journal of Clinical Pathology·Ana Mafalda PintoOlga Maria Lage
Sep 20, 2018·The Journal of Obstetrics and Gynaecology Research·Hwan Tae LeeYiel-Hea Seo
Apr 25, 2009·Acta Microbiologica Et Immunologica Hungarica·Katalin KristófK Nagy
May 22, 2019·BMC Pregnancy and Childbirth·Kate ClouseNatasha Halasa

❮ Previous
Next ❯

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.

Related Papers

Clinical Microbiology and Infection : the Official Publication of the European Society of Clinical Microbiology and Infectious Diseases
M ConvertJ-C Piffaretti
Clinical Infectious Diseases : an Official Publication of the Infectious Diseases Society of America
H Dele DaviesJeanne A Jordan
© 2021 Meta ULC. All rights reserved