Sensitivities of PCR, MicroTrak, ChlamydiaEIA, IDEIA, and PACE 2 for purified Chlamydia trachomatis elementary bodies in urine, peripheral blood, peripheral blood leukocytes, and synovial fluid.

Journal of Clinical Microbiology
J G KuipersH Zeidler

Abstract

Routine microbiological diagnosis of Chlamydia-induced reactive arthritis is based mainly on the detection of Chlamydia trachomatis with urogenital swabs or in urine. Because chlamydial antigen, rRNA, and DNA are present in low quantities in the inflamed joint, highly sensitive assays are needed to detect C. trachomatis not only at the primary site of infection but also in peripheral blood and peripheral blood leukocytes, which are suspected carriers for dissemination, and in synovial fluid. To evaluate possible tools for this purpose, the sensitivities of PCR, MicroTrak, Chlamydia EIA, IDEIA, and PACE 2 for the detection of defined numbers of purified C. trachomatis elementary bodies (EB) in urine, peripheral blood, peripheral blood leukocytes, and synovial fluid were determined. In urine, PCR detected 2, MicroTrak and ChlamydiaEIA detected 2 x 10(3), and PACE 2 and IDEIA detected 2 x 10(4) EB per ml. In peripheral blood, only PCR and MicroTrak detected C. trachomatis, with detection limits of 100 and 2 x 10(7) EB per ml, respectively. For peripheral blood leukocytes, the detection limits were 2 EB per ml for PCR and 2 x 10(4) EB per ml for MicroTrak, ChlamydiaEIA, IDEIA, and PACE 2. In synovial fluid, PCR detected 200, MicroT...Continue Reading

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Citations

Aug 30, 2005·Human Reproduction·Annalisa SattaAldo E Calogero
Jul 15, 2015·Zeitschrift für Rheumatologie·J FreiseJ G Kuipers
Nov 1, 2003·Rheumatology International·Michael C JendroMichael Martin
Jun 9, 2009·Journal of Clinical Rheumatology : Practical Reports on Rheumatic & Musculoskeletal Diseases·Markus RihlHenning Zeidler
Feb 16, 2010·Arthritis and Rheumatism·Markus RihlHenning Zeidler
Oct 19, 2004·Clinical Microbiology Reviews·Anthony W SolomonDavid C W Mabey
Apr 9, 2010·International Journal of Rheumatic Diseases·Mohammad-Bagher Owlia, Adrian R Eley
Nov 17, 2005·Rheumatic Diseases Clinics of North America·Helena Marzo-OrtegaMichael J Green

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