PMID: 9002137Jan 1, 1996Paper

Sensitivity and predictability of vitreous cytology, biopsy, and membrane filter culture in endophthalmitis

Retina
S SharmaT Das

Abstract

Forty-seven consecutive patients with endophthalmitis were prospectively studied to: 1) compare the undiluted vitreous biopsy culture and membrane filter culture of vitrectomy cassette fluid; 2) determine the sensitivity and specificity of initial smear examinations in relation to final culture; and 3) characterize vitreous cytology in culture positive and negative endophthalmitis. Examinations included smears (Gram stains, Diff Quik [Bacto Laboratories Pvt. Ltd., Liverpool, Australia] and unstained) and aerobic/anaerobic culture of undiluted vitreous biopsy specimens and diluted vitreous collected in the cassette. The cassette fluid was passed through 5-microns polycarbonate filter for cytology and a 0.22-microns polyvinylidene difluoride millipore filter for culture. By either method culture results were positive in 27 (57.4%) patients. Culture positive of the vitreous biopsy alone was 44.6% and cassette fluid alone was 49% (P > 0.05). In the initial smear examination the sensitivity and specificity of the Gram stain were 66.6% and 84.2%, respectively. comparatively, Diff Quik provided lower sensitivity and specificity at 40.7% and 80.9% respectively. Examination of wet film of vitreous samples did not provide any useful info...Continue Reading

Citations

Oct 19, 2007·Der Ophthalmologe : Zeitschrift der Deutschen Ophthalmologischen Gesellschaft·T Ness
Oct 3, 2001·Ophthalmology·T DasUNKNOWN Endophthalmitis Research Group
Mar 8, 2003·Ophthalmology·Aires Lobo, Susan Lightman
Jun 14, 2006·International Ophthalmology Clinics·Rishi P Singh, Lucy H Young
Jun 10, 2004·Retina·Rakesh GuptaUNKNOWN Endophthalmitis Research Group
Apr 5, 2005·Retina·Herminia Miño de KasparAnselm Kampik
Sep 12, 2006·Retina·Mehran TabanJonathan E Sears
Apr 21, 2010·Indian Journal of Medical Microbiology·S Sharma
Apr 24, 2010·Indian Journal of Ophthalmology·Suma NalamadaAshok Kumar Reddy
Mar 15, 2011·Survey of Ophthalmology·Neelakshi BhagatMarco Zarbin
Feb 1, 2011·Ophthalmology·Francis Char DeCroosUNKNOWN Hyderabad Endophthalmitis Research Group
Oct 10, 2009·Ophthalmology·Christophe ChiquetUNKNOWN French Institutional Endophthalmitis Study (FRIENDS) group
Apr 3, 2007·Journal of Cataract and Refractive Surgery·Christophe ChiquetFrançois Vandenesch
Jun 13, 2006·American Journal of Ophthalmology·Assaf KratzTova Lifshitz
Dec 24, 2014·Journal of Cataract and Refractive Surgery·Savitri SharmaSuryasnata Rath
Jan 15, 2020·Asia-Pacific Journal of Ophthalmology·Vivek Pravin DaveTaraprasad Das
Sep 5, 2006·European Journal of Ophthalmology·J G GarwegM Halberstadt
Dec 21, 2019·Indian Journal of Ophthalmology·Vivek Pravin DaveTaraprasad Das
Apr 23, 2020·Indian Journal of Ophthalmology·Vivek Pravin DaveTaraprasad Das
Dec 16, 1998·The Japanese Journal of Thoracic and Cardiovascular Surgery : Official Publication of the Japanese Association for Thoracic Surgery = Nihon Kyōbu Geka Gakkai Zasshi·S KawaguchiM Ishikawa
Oct 12, 2018·Journal of Ophthalmic Inflammation and Infection·Joveeta JosephVivek Pravin Dave
Jan 29, 2020·Asia-Pacific Journal of Ophthalmology·Vivek Pravin DaveTaraprasad Das
Oct 30, 2004·Seminars in Ophthalmology·Taraprasad DasUNKNOWN Hyderabad Endophthalmitis Research Group
Feb 13, 2021·The British Journal of Ophthalmology·Loic LevezielVincent Borderie
Nov 27, 2019·American Journal of Ophthalmology·Julie KosackiUNKNOWN Friends Group
Jun 3, 2021·Clinical Microbiology Reviews·Sixto M LealPeter H Gilligan

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