Sensitivity and specificity of enzyme immunoassay for serodiagnosis of influenza A virus infections

The Journal of Infectious Diseases
G W HammondG R Noble

Abstract

Antibodies to influenza virus in sera from 40 patients infected with influenza A/USSR/90/77H1N1-like virus were measured by an enzyme immunoassay (EIA), and results were compared with those obtained by complement-fixation (CF) and hemagglutination-inhibition (HAI) tests. The sensitivity of EIA in detecting an increase in influenza antibody in sera from these 40 patients was intermediate (27 of 40) between the CF test (19 of 40) and the HAI test (35 of 40) when an ether-treater influenza A/USSR/77 virus was used as antigen in all three tests. In contrast with results from HAI tests, however, EIA did not reliably distinguish between infections caused by H1N1 and H3N2 viruses; EIA was thus most comparable in specificity to the CF test. It appears, therefore, that both EIA and CF tests measure antibodies directed to internal antigens common to all type A influenza strains, and that EIA with whole or ether-split influenza virus antigen may be a feasible alternative to the CF test for the type-specific serodiagnosis of influenza infections.

Citations

Dec 1, 1992·Toxicon : Official Journal of the International Society on Toxinology·N H TanM I Jaafar
Aug 1, 1982·Journal of Virological Methods·E N Al-Kaissi, A Mostratos
Aug 1, 1987·Epidemiology and Infection·P KoskinenO Meurman
Apr 20, 2012·PloS One·Brendan KlickBenjamin J Cowling
Jul 1, 1988·Journal of Medical Virology·J C KingK M Edwards
Jan 1, 1988·Scandinavian Journal of Infectious Diseases. Supplementum·K Lehtomäki
Jun 23, 2011·Expert Review of Anti-infective Therapy·Jacqueline M KatzXiyan Xu
Feb 23, 2020·Viruses·Daniel KiselevKamil Khafizov
Jan 1, 1981·Journal of Clinical Microbiology·A S Monto, H F Maassab
Mar 1, 1989·Journal of Clinical Microbiology·T VikerforsJ van der Logt

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