Sensitivity and Specificity of Gene Xpert in the Diagnosis of Spinal Tuberculosis: A Prospective Controlled Clinical Study

Global Spine Journal
Anil M SolankiAditya Banta

Abstract

Prospective matched cohort study. To evaluate the sensitivity and specificity of Gene Xpert in the diagnosis of spinal tuberculosis. From January 2016 to August 2018, Gene Xpert results were prospectively studied in 68 patients of clinicoradiologically suspected spinal tuberculosis (STB) and a control group (CG) of 92 patients, all of whom underwent computed tomography-guided/C-arm-guided/open surgical biopsy. Sensitivity, specificity, positive predictive value, and negative predictive value are obtained using standard equations. Out of 68 cases of STB, Gene Xpert was positive in 62 (true positive: 62/68) and negative in 6 (false negative: 6/68). Gene Xpert was negative for all 92 cases of CG (true negative: 92/92, false positive 0/92). Thus, in our series, sensitivity of Gene Xpert is 91.18%, specificity is 100%, positive predictive value is 100%, and negative predictive value is 93.88%. Out of all cases of STB, 62/68 (91.18%) were Gene Xpert positive, but only 35/64 (54.69%) was acid-fast bacilli (AFB) culture positive and 53/60(88.33%) was histopathologically conclusive of TB. Also, Gene Xpert was positive in 7/7 (100%) cases of STB in which histopathology were inconclusive and 25/29 (86.21%) cases of STB in which AFB cultur...Continue Reading

References

Feb 1, 1994·The Journal of Bone and Joint Surgery. American Volume·A Mondal
Sep 15, 1999·Acta Radiologica·M KangS Suri
May 3, 2003·The Lancet Infectious Diseases·Kamran SiddiqiJohn Walley
Sep 8, 2005·Journal of Clinical Microbiology·Soumitesh ChakravortyJaya Sivaswami Tyagi
May 28, 2010·Journal of Clinical Microbiology·Robert BlakemoreDavid Alland
Sep 10, 2010·The New England Journal of Medicine·Catharina C BoehmeMark D Perkins
Sep 10, 2010·The New England Journal of Medicine·Peter M Small, Madhukar Pai
Nov 28, 2012·The European Respiratory Journal·Karin WeyerMario Raviglione
Nov 30, 2012·Respirology : Official Journal of the Asian Pacific Society of Respirology·Keertan DhedaWing Cheong Yam
Nov 8, 2013·Journal of Clinical Microbiology·Nguyen Thi Quynh NhuMaxine Caws
Apr 4, 2014·The European Respiratory Journal·Claudia M DenkingerKaren R Steingart
Mar 12, 2016·International Journal of Mycobacteriology·Asma GharianiLeila Slim-Saidi
Apr 10, 2017·European Spine Journal : Official Publication of the European Spine Society, the European Spinal Deformity Society, and the European Section of the Cervical Spine Research Society·Justin ArockiarajVenkatesh Krishnan

❮ Previous
Next ❯

Citations

Jan 19, 2021·International Journal of Infectious Diseases : IJID : Official Publication of the International Society for Infectious Diseases·Luxia KongShuyun Xu
Mar 9, 2021·Journal of Clinical Orthopaedics and Trauma·Vijay KarthekSiddharth N Aiyer
Mar 5, 2021·Current Opinion in Neurology·Sofiati DianArjan van Laarhoven

❮ Previous
Next ❯

Methods Mentioned

BETA
biopsy
nucleic acid amplification
PCR

Software Mentioned

Gene Xpert

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.