Sensitivity and specificity of standardised allergen extracts in skin prick test for diagnoses of IgE-mediated respiratory allergies

Clinical and Translational Allergy
Nicola Wagner, Michael Rudert

Abstract

Skin prick tests (SPTs) are essential for the diagnosis of IgE-mediated allergy and are influenced by extract quality, biological potency and concentration of allergen. In this open multicentre study 431 patients, aged 18-64 years were enrolled. Patients had a history of IgE-mediated allergy and a sensitisation (previous positive SPT of any manufacturer) against at least one of the investigated allergens: 6-grass pollen, house dust mite, birch and mugwort pollen. In our study, these allergens were tested in five concentrations each. To establish the optimal trade-off between sensitivity and specificity, the area under the receiver operating characteristic (ROC) curve was estimated by comparing the outcome of the SPT with three methods referred to as 'reference methods' (specific IgE, clinical case history and a previous SPT). For all allergens and reference methods, the area under the ROC curves were highly significant (p < 0.001). Specific IgE reference method resulted in the largest area under the curve (AUC) for all allergens (0.80-0.90) followed by previous SPT (0.70-0.87) and case history (0.65-0.74). Sensitivity of SPT increased with increasing concentration and specificity decreased. For all allergens, compared to specif...Continue Reading

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Citations

Nov 12, 2020·Clinical and Experimental Allergy : Journal of the British Society for Allergy and Clinical Immunology·Ekaterina PotapovaPaolo Maria Matricardi
Dec 10, 2020·Clinical and Translational Allergy·J BousquetM Jutel
Dec 10, 2020·Clinical and Translational Allergy·Lan ZhaoRonald van Ree
Oct 8, 2020·The World Allergy Organization Journal·Todor A PopovIgnacio J Ansotegui

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