Sensitivity and specificity of the agar-gel-immunodiffusion test, ELISA and the skin test for detection of paratuberculosis in United States Midwest sheep populations

Veterinary Research
Suelee Robbe-AustermanChad Ewing

Abstract

Our objective was to estimate the sensitivity and specificity of the agar-gel-immunodiffusion test (AGID), the ELISA, and the skin test for the detection of Mycobacterium avium subspecies paratuberculosis (MAP) in sheep using Bayesian methods without a gold standard. Fourteen flocks (2 465 sheep) were used. Five flocks (450 sheep) were considered MAP non-infected and 9 flocks (2 015 sheep) had sheep infected with MAP. Sheep were skin tested and blood was collected for AGID and ELISA testing. Results were analyzed using a Bayesian 3-test in 1-population model fitted in WinBUGS. The model allowed for dependence (correlation) between the two serologic tests, but these two tests were assumed to be conditionally independent of the skin test. The estimated specificity was 99.5% (95% PI of 98.9-99.9%) for the AGID; 99.3% (98.4-99.8%) for the ELISA using an optical density measured cutoff of 0.20; 99.2% (98.1-99.8%) using a cutoff of 0.15; 97.5% (95.8-98.7%) using a cutoff of 0.10; and 98.7% (97.3-99.5%) for the skin test. The estimated sensitivities were 8.3% (6.2-10.7%) for the AGID; 8.0% (6.0-10.4%), 10.6% (8.3-13.1%), and 16.3% (13.5-19.4%) for the ELISA using the cutoffs 0.20, 0.15, and 0.10 respectively; and 73.3% (62.3-85.8%) fo...Continue Reading

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Citations

Oct 30, 2016·The Veterinary Quarterly·Kundan Kumar ChaubeyAbdollah Derakhshandeh
Apr 20, 2017·Journal of Immunoassay & Immunochemistry·Elizama Ponce BarrazaSawako Hori-Oshima
Feb 6, 2007·Veterinary Immunology and Immunopathology·A J NedrowM L Thonney
Apr 27, 2007·Journal of Veterinary Diagnostic Investigation : Official Publication of the American Association of Veterinary Laboratory Diagnosticians, Inc·Suelee Robbe-AustermanDaniel G Morrical
Jun 20, 2013·Journal of Veterinary Diagnostic Investigation : Official Publication of the American Association of Veterinary Laboratory Diagnosticians, Inc·Sachin KumthekarHarry Hariharan
Aug 30, 2014·Tropical Animal Health and Production·Jorge Arturo Fernández-SilvaNicolás Fernando Ramírez
Sep 9, 2006·Clinical and Vaccine Immunology : CVI·Makeda SemretMarcel A Behr
Aug 8, 2015·Tropical Animal Health and Production·Alessia GalieroDomenico Cerri

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