Sensitivity and specificity of various serological tests for the detection of Toxoplasma gondii infection in naturally infected sheep

Veterinary Parasitology
R M ShaapanM A A Tawfik

Abstract

Comparative serological examination of 300 serum samples from sheep slaughtered in the main abattoir in Cairo, Egypt revealed a higher prevalence of toxoplasmosis (43.7%) with the modified agglutination test (MAT), followed by the enzyme linked immune-sorbant assay (ELISA) (41.7%) and the indirect fluorescent antibody test (IFAT) (37%), while the lowest prevalence was detected with the dye test (DT) (34%). When the data from the first three serological tests were compared with that of the DT test, which was used as a reference test for toxoplasmosis, MAT had the highest sensitivity (96%), followed by ELISA (90.1%) and IFAT, which demonstrated the lowest sensitivity (80.4%). Conversely, IFAT had the highest specificity (91.4%), followed by MAT (88.9%) and ELISA (85.9%).

References

Jan 1, 1975·Transactions of the Royal Society of Tropical Medicine and Hygiene·M A RifaatS M Hanna
Aug 1, 1992·Clinical Infectious Diseases : an Official Publication of the Infectious Diseases Society of America·B J Luft, J S Remington
Sep 1, 1987·Australian Veterinary Journal·B L MundayR W Mason
Mar 1, 1987·The British Veterinary Journal·D A Blewett, A J Trees
Jul 1, 1987·Equine Veterinary Journal·J P Dubey, G Desmonts
Jan 1, 1973·Transactions of the Royal Society of Tropical Medicine and Hygiene·A J SulzerI G Kagan
Feb 1, 1965·Proceedings of the Society for Experimental Biology and Medicine·G L STEWART, H A FELDMAN
Apr 22, 2004·Transactions of the Royal Society of Tropical Medicine and Hygiene·B A BotrosR R Graham

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Citations

Jul 30, 2014·Veterinary Parasitology·D VerhelstE Cox
Feb 9, 2013·BMC Veterinary Research·Beniamino T Cenci-GogaPeter N Thompson
Apr 25, 2013·PloS One·Vignesh R PuvanesuaranVenugopal Balakrishnan
May 18, 2013·Avian Diseases·Vignesh R PuvanesuaranVenugopal Balakrishnan
Oct 30, 2013·Pakistan Journal of Biological Sciences : PJBS·Ashraf Mohamed BarakatEhab Kotb El-Mahllawy
Apr 14, 2011·Foodborne Pathogens and Disease·Beniamino T Cenci-GogaJames S Cullor
Feb 11, 2010·Veterinary Parasitology·Sam MasonJudith E Smith
Nov 17, 2015·Animal Health Research Reviews·Heidi M Wyrosdick, John J Schaefer
May 29, 2015·Parasites & Vectors·Quan LiuXing-Quan Zhu
Apr 17, 2016·BMC Infectious Diseases·Aisha GashoutAbdalhafid Abudher
Aug 26, 2011·Veterinary Parasitology·Isabelle VillenaLénaïg Halos
Apr 28, 2009·Veterinary Parasitology·J P Dubey
Feb 18, 2014·International Journal for Parasitology. Parasites and Wildlife·Tracey HollingsHamish McCallum
Oct 17, 2012·Revista brasileira de parasitologia veterinária = Brazilian journal of veterinary parasitology : Órgão Oficial do Colégio Brasileiro de Parasitologia Veterinária·Francine Bragagnolo Liz Stefen SakataJuliana Antunes Farias
Jul 23, 2015·Journal of Veterinary Diagnostic Investigation : Official Publication of the American Association of Veterinary Laboratory Diagnosticians, Inc·Yi GuQuan Liu
Apr 3, 2018·BMC Veterinary Research·Yara M Al-KappanyEric Cox
Feb 16, 2019·Parasite : Journal De La Société Française De Parasitologie·Mariem RouatbiMohamed Gharbi
Dec 8, 2009·The Korean Journal of Parasitology·Juan Hua QuanYoung-Ha Lee
Sep 29, 2019·Parasitology·I E AbbasJ P Dubey
Jan 6, 2009·The Journal of Eukaryotic Microbiology·Jitender P Dubey
Mar 27, 2015·Parasite : Journal De La Société Française De Parasitologie·Abdelkrim AroussiDaniel Ajzenberg
Nov 24, 2016·Journal of Parasitic Diseases : Official Organ of the Indian Society for Parasitology·Raafat Mohamed Shaapan
Nov 8, 2017·Asian Pacific Journal of Tropical Medicine·Hany M IbrahimHend E El-Shqanqery
Mar 28, 2017·Veterinary World·Aretas Babatoundé Nounnagnon TonouhewaSouaïbou Farougou
Feb 7, 2019·Pakistan Journal of Biological Sciences : PJBS·Nawal Abdel Hafez HassanainMohey Abdel Hafez Hassanain
Dec 13, 2018·Journal of Parasitic Diseases : Official Organ of the Indian Society for Parasitology·Khaled A Abd El-RazikYousef A Soliman
May 28, 2020·Frontiers in Cellular and Infection Microbiology·Rochelle Haidee D YbañezYoshifumi Nishikawa

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