Sensitivity enhancement in 1D heteronuclear NMR spectroscopy via single-scan inverse experiments

Chemphyschem : a European Journal of Chemical Physics and Physical Chemistry
Mor Mishkovsky, Lucio Frydman

Abstract

Measuring the nuclear magnetic resonance spectra of low-gamma heteronuclei such as 15N constitutes an important analytical tool for the characterization of molecular structure and dynamics. The reduced resonance frequencies and magnetic moments of these heteronuclei, however, make the sensitivity of this kind of spectroscopy inherently lower than that of comparable H NMR observations. A well-known solution to this sensitivity problem is indirect detection: a 2D NMR technique capable of enhancing the sensitivity of heteronuclear NMR by porting the actual data acquisition from the low-gamma nucleus to neighboring protons. This has become the standard method of observation in biomolecular NMR, where the resolution introduced by 2D spectroscopy is always a sought-after commodity. Indirect detection, however, has not gained a wide appeal in organic chemistry or in in vivo investigations, where one-dimensional heteronuclear NMR information usually suffices. The present study explores the possibility of retaining certain advantages derived from indirect detection while not giving up on the simple one-dimensional nature of heteronuclear NMR, by relying on the spatial-encoding scheme we have recently demonstrated for implementing single...Continue Reading

Citations

Nov 1, 2011·Journal of Biomolecular NMR·Maayan GalGerhard Wagner
Aug 8, 2015·Magnetic Resonance in Chemistry : MRC·Maayan Gal, Lucio Frydman

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