Sensitivity of Escherichia coli O157 detection in bovine feces assessed by broth enrichment followed by immunomagnetic separation and direct plating methodologies

Journal of Clinical Microbiology
Jeffrey T LeJeuneThomas E Besser

Abstract

In order to more precisely predict food safety risks, the fecal presence of food-borne pathogens among animals at slaughter must be correctly determined. Quantification of Escherichia coli O157 is also desirable. In two separate experiments, detection and enumeration of a nalidixic acid-resistant strain of E. coli O157 in bovine feces was assessed by culture on MacConkey agar supplemented with nalidixic acid (MACnal) and compared to overnight broth enrichment followed by immunomagnetic separation (IMS) and to direct plating of dilutions of bovine feces onto sorbitol MacConkey agar containing cefixime and tellurite (SMACct). The sensitivity of detection of E. coli O157 by both direct plating and IMS was highly dependent upon the initial concentration of the target organism in the sample. Sensitivity of detection by IMS was poor below 100 CFU/g but was better, and not affected by initial E. coli O157 numbers, above this concentration. Sensitivity of detection of E. coli O157 in bovine feces at low initial concentrations is very poor for both direct plating and IMS. Direct plating of dilutions of bovine feces on SMACct can be used to determine the magnitude of fecal E. coli excretion among cattle excreting greater than 100 CFU/g. ...Continue Reading

References

Oct 1, 1995·Journal of Clinical Microbiology·M W SandersonT E Besser
Jul 1, 1996·Applied and Environmental Microbiology·G WangM P Doyle
Jul 23, 1999·Preventive Veterinary Medicine·D JordanJ B Wilson
Feb 9, 2000·Journal of Applied Microbiology·Y HuJ C Meitzler
Mar 22, 2000·Proceedings of the National Academy of Sciences of the United States of America·R O ElderW W Laegreid
May 1, 2001·Letters in Applied Microbiology·J T LejeuneD D Hancock
Jan 7, 2003·Applied and Environmental Microbiology·Elina LahtiTuula Honkanen-Buzalski
May 7, 2003·Applied and Environmental Microbiology·F OmisakinN J C Strachan
Oct 14, 2004·Journal of Applied Microbiology·S E RobinsonN P French

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Citations

Mar 19, 2009·Foodborne Pathogens and Disease·P AyscueY T Gröhn
Dec 2, 2010·Foodborne Pathogens and Disease·Witold A Ferens, Carolyn J Hovde
Jun 19, 2007·Applied and Environmental Microbiology·J Trent FoxT G Nagaraja
Nov 1, 2011·Applied and Environmental Microbiology·Ursula RajtakSéamus Fanning
Nov 7, 2006·Journal of Animal Science·C L Gyles
Feb 2, 2008·Journal of Food Protection·Michael A Grant
Nov 20, 2018·International Journal of Food Microbiology·Luca RotundoGeorge Paoli
Nov 1, 2017·Comprehensive Reviews in Food Science and Food Safety·Vinicius Silva CastroEduardo Eustáquio Souza Figuiredo
Mar 19, 2020·Journal of Microbiological Methods·Jeffrey C ChandlerSusan A Shriner

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