PMID: 891549Aug 1, 1977Paper

Sensitivity of hemoglobin thiol groups within red blood cells of rat during oxidation of glutathione

European Journal of Biochemistry
N S KosowerR L Koppel

Abstract

The intracellular thiol-oxidising diazenes, diazenedicarboxylic acid bis-N,N-dimethylamide and diazenedicarboxylic acid bis-N'-ethylpiperazinide, have been used in the study of red cells. A difference in the consequences of diazene oxidant treatment between the human red cell and rat red cells has been found in respect to the quantity of oxidant needed for glutathione (GSH) oxidation, to the fate of GSH, and to the reactivity of hemoglobin. In the first place, significantly more oxidant is needed for GSH oxidation in the rat red cell than in the human cell. Secondly, in the human cell, all of the GSH is converted to glutathione disulfide (GSSG), from which GSH is regenerated. In the rat cell, GSH disappears without being converted to GSSG, and GSH is not regenerated. Thirdly, a decrease in rat hemoglobin thiol groups, but no change in human hemoglobin, is found. Sterically unhindered thiol groups in the rat hemoglobin are thought to react with the usual adduct intermediate in GSH oxidation by diazene (formed from RCON = NCOR + GSH leads to RCON(SG)NHCOR) to produce mixed disulfides, from which GSH is not easily regenerated. The results support the idea that reduction of mixed disulfides of GSH and protein is not carried out dir...Continue Reading

References

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Citations

Jan 1, 1979·Pharmacology & Therapeutics·J W Harris
Jan 1, 1987·Comparative Biochemistry and Physiology. B, Comparative Biochemistry·M Erden
Jan 1, 1990·Comparative Biochemistry and Physiology. B, Comparative Biochemistry·A L Dafré, E Reischl
Aug 1, 1992·Comparative Biochemistry and Physiology. B, Comparative Biochemistry·E Reischl, A L Dafré
Jan 1, 1996·Comparative Biochemistry and Physiology. Part B, Biochemistry & Molecular Biology·Y EnokiM Hattori
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Apr 21, 2006·Biochemical Pharmacology·D GiustariniRanieri Rossi
Oct 12, 2000·Comparative Biochemistry and Physiology. Part B, Biochemistry & Molecular Biology·M A Torsoni, S H Ogo
Feb 28, 2001·Applied Radiation and Isotopes : Including Data, Instrumentation and Methods for Use in Agriculture, Industry and Medicine·S SeifertB Johannsen
Jul 1, 1979·Proceedings of the National Academy of Sciences of the United States of America·N S KosowerH M Ranney
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Feb 6, 1981·Biochimica Et Biophysica Acta·N S KosowerR Shomrat
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