Sensitivity of immunoaffinity-purified porcine 5-lipoxygenase to inhibitors and activating lipid hydroperoxides

Biochemical Pharmacology
D RiendeauS Yamamoto

Abstract

The requirement for hydroperoxide activation and the effect of inhibitors from different structural classes on 5-lipoxygenase activity were determined on the immunoaffinity-purified enzyme from porcine leukocytes. The 5-lipoxygenase activity was measured using a continuous spectrophotometric assay monitoring the increase in conjugated diene formation (A235) upon incubation of the enzyme with arachidonic acid. Under standard assay conditions, the reaction progress curves showed little or no lag phase, with a rapid first-order decay in enzyme activity (T1/2 = 0.7 to 1.1 min). Both the initial rate of the reaction and total product formation were stimulated by the addition of ATP, Ca2+ and phosphatidylcholine (PC). PC (24 micrograms/ml) was also found to increase the recovery of radiolabeled arachidonic acid from the assay mixture and thus part of the stimulation may be due to an increase in substrate availability and reduction of surface adsorption effects. The requirement of hydroperoxides for the initiation of the reaction was shown by the induction of 0.1 to 1-min lag phases using NaBH4 or glutathione peroxidase and by the reduction in lag times by 5-hydroperoxyeicosatetraenoic acid (5-HPETE) and 13-hydroperoxyoctadecadienoic ...Continue Reading

References

Nov 1, 1975·Canadian Journal of Biochemistry·H W Cook, W E Lands
Apr 9, 1985·Biochemistry·C H ClappS A Rotenberg
Jun 1, 1986·Proceedings of the National Academy of Sciences of the United States of America·T ShimizuB Samuelsson
Mar 1, 1986·Prostaglandins·M T SkoogJ S Wiseman
Mar 1, 1986·Prostaglandins·J L BeneytoutM Tixier
Dec 15, 1986·Biochemical and Biophysical Research Communications·D Riendeau, Y Leblanc
Jan 15, 1988·Biochemical and Biophysical Research Communications·E SigalJ A Nadel
Jan 1, 1988·Annals of the New York Academy of Sciences·C A RouzerH G Bull
Dec 1, 1987·Canadian Journal of Physiology and Pharmacology·L Y Choo, D Riendeau
Mar 1, 1987·Journal of Medicinal Chemistry·J B SummersG W Carter
Sep 1, 1985·Proceedings of the National Academy of Sciences of the United States of America·C A Rouzer, B Samuelsson
Oct 15, 1982·Biochemical Pharmacology·A M Hanel, W E Lands

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Citations

May 1, 1996·Inflammation Research : Official Journal of the European Histamine Research Society ... [et Al.]·F CabréG Carganico
Mar 20, 2009·Canadian Journal of Anaesthesia = Journal Canadien D'anesthésie·Hui-Jin SungKoji Ogawa
Nov 16, 2002·Prostaglandins & Other Lipid Mediators·Olof Rådmark
Jan 1, 1992·Proceedings of the National Academy of Sciences of the United States of America·C P Sparrow, J Olszewski
Jan 15, 1996·European Journal of Biochemistry·E De CarolisD Riendeau
Feb 15, 2000·American Journal of Respiratory and Critical Care Medicine·O P Rådmark
Feb 26, 2009·International Immunopharmacology·C A C Hyde, S Missailidis
Feb 24, 1993·Biochemical Pharmacology·J P FalgueyretD Riendeau
Dec 1, 1993·Prostaglandins, Leukotrienes, and Essential Fatty Acids·J BretonD Griswold
Aug 27, 2005·Biochemical and Biophysical Research Communications·Olof Rådmark, Bengt Samuelsson
Apr 29, 2015·Free Radical Biology & Medicine·Josefin ZschalerJürgen Arnhold
Jan 15, 2002·American Journal of Physiology. Heart and Circulatory Physiology·Jennifer L WalkerYing-Yi Zhang
Sep 10, 2017·Scientific Reports·Carlos A SorgiLucia H Faccioli
Aug 15, 1997·Biochemical Pharmacology·H SuzukiS Yamamoto

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