Sensitivity of isoenzyme analysis for the detection of interspecies cell line cross-contamination

In Vitro Cellular & Developmental Biology. Animal
R W NimsJ W Harbell

Abstract

The analysis of the gel electrophoresis banding patterns and relative migration distances for the individual isoforms of intracellular enzymes, such as lactate dehydrogenase, purine nucleoside phosphorylase, glucose-6-phosphate dehydrogenase, and malate dehydrogenase, is used routinely in the biopharmaceutical industry for confirmation of cell line species of origin. In the present study, the sensitivity of the technique (AuthentiKit, Innovative Chemistry, Marshfield, MA) for determining interspecies cell line cross-contamination was examined. Extracts were prepared from a CHO-K1 line (AA8, Chinese hamster), MRC-5 (human) cells, and L929 (mouse) cells and from several proportional mixtures of the various binary combinations of cells. The isoenzymes were analyzed according to standard procedures for the technique. Contamination of MRC-5 cells with CHO-K1 or with L929 cells was clearly detectable with each enzyme analyzed. Similarly, the contamination of L929 or CHO-K1 cells with MRC-5 cells was readily apparent with each enzyme. On the other hand, contamination of CHO-K1 cells with L929 cells was only detected with lactate dehydrogenase analysis, and contamination of L929 cells with CHO-K1 cells was not detected with any of the ...Continue Reading

References

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Citations

Nov 13, 2008·Cytotechnology·C M CabreraA Concha
Mar 6, 2009·In Vitro Cellular & Developmental Biology. Animal·Lin-feng LiYue-hui Ma
Oct 12, 2010·In Vitro Cellular & Developmental Biology. Animal·Raymond W NimsEugene Elmore
Jun 21, 2001·Proceedings of the National Academy of Sciences of the United States of America·J R MastersP G Debenham
Jun 21, 2008·Acta Pharmacologica Sinica·Armando RojasHéctor Figueroa
Mar 12, 2011·Regenerative Medicine·Liz Kerrigan, Raymond W Nims
Jul 4, 2012·Viruses·Ingo JordanVolker Sandig
May 11, 2011·European Journal of Histochemistry : EJH·C BaiY Ma
Jul 30, 2014·Experimental Hematology·Maria Bach LaursenUNKNOWN Myeloma Stem Cell Network
May 23, 2007·In Vitro Cellular & Developmental Biology. Animal·Kazumi OnoHidekazu Sawada
Mar 20, 2010·Artificial Cells, Blood Substitutes, and Immobilization Biotechnology·Chunyu BaiQianjun Zhao
Jan 10, 2012·Methods : a Companion to Methods in Enzymology·Gargi Seth
Jun 27, 2008·Tissue & Cell·Changqing LiuXuexi Tang
Jan 27, 2007·Biologicals : Journal of the International Association of Biological Standardization·Shahram AzariFazel Shokri
Jul 7, 2011·Animal Science Journal = Nihon Chikusan Gakkaihō·Chang-Qing LiuYue-Hui Ma
Oct 26, 2018·Journal of Cellular and Molecular Medicine·Jun LinWeiwen Cai
May 8, 2010·Nature Reviews. Cancer·UNKNOWN American Type Culture Collection Standards Development Organization Workgroup ASN-0002
May 29, 2015·Nature Methods·Leonard P FreedmanYvonne A Reid
Oct 17, 2019·Anais Da Academia Brasileira De Ciências·Shirley B DE AraújoMaria Judite B Fernandes

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