Sensitivity of notochordal disc cells to mechanical loading: an experimental animal study.

European Spine Journal : Official Publication of the European Spine Society, the European Spinal Deformity Society, and the European Section of the Cervical Spine Research Society
Thorsten GuehringGeorg W Omlor

Abstract

The immature disc nucleus pulposus (NP) consists of notochordal cells (NCs). With maturation NCs disappear in humans, to be replaced by chondrocyte-like mature NP cells (MNPCs); this change in cell phenotype coincidences with early signs of disc degeneration. The reasons for NC disappearance are important to understand disc degeneration, but remain unknown, yet. This study investigated, whether loading induced a change from a notochordal nucleus phenotype to a chondrocyte-like one. An in vivo disc compression model with fixateur externe was used in 36 mature rabbits. Discs were compressed for different time periods (1, 28, 56 days), and compared with uncompressed control discs (56 days without treatment), and discs with sham compression (28 days). Nucleus cell phenotype was determined by histology and immunohistochemistry. NCs, but not MNPCs highly expressed bone-morphogenetic-protein 2 and cytokeratin 8, thus NC and MNPC numbers could be determined. A histologic score was used to detect structural endplate changes after compression (28 days). Control and sham compressed discs contained around 70% NCs and 30% MNPCs, to be decreased to <10% NCs after 28-56 days of loading. NC density fell sharply by >50% after 28-56 days of comp...Continue Reading

References

Jan 1, 1981·Connective Tissue Research·S HolmA Nachemson
Mar 21, 1998·Journal of the American Animal Hospital Association·J P Bray, H M Burbidge
May 14, 1998·Clinical Anatomy : Official Journal of the American Association of Clinical Anatomists & the British Association of Clinical Anatomists·S ChandrarajK Opeskin
Jan 12, 1999·Experimental Cell Research·D J AguiarT R Oegema
Apr 18, 2001·Developmental Dynamics : an Official Publication of the American Association of Anatomists·Y ZhuL J Sandell
Nov 21, 2002·Biochemical Society Transactions·A McAlindenL J Sandell
Nov 21, 2002·Biochemical Society Transactions·T R Oegema
Dec 6, 2003·Journal of Orthopaedic Research : Official Publication of the Orthopaedic Research Society·Erika I Palmer, Jeffrey C Lotz
Nov 27, 2004·Spine·Jill P G UrbanJeremy C T Fairbank
Nov 27, 2004·Spine·Lori A Setton, Jun Chen
Feb 18, 2005·European Spine Journal : Official Publication of the European Spine Society, the European Spinal Deformity Society, and the European Section of the Cervical Spine Research Society·Frank UnglaubMarkus W Kroeber
Sep 1, 2005·European Spine Journal : Official Publication of the European Spine Society, the European Spinal Deformity Society, and the European Section of the Cervical Spine Research Society·Thorsten GuehringMarkus W Kroeber
Feb 16, 2006·Journal of Orthopaedic Research : Official Publication of the Orthopaedic Research Society·Georg W OmlorThorsten Guehring
Apr 6, 2006·The Journal of Bone and Joint Surgery. American Volume·Thijs GrunhagenJill P G Urban
Mar 6, 2007·Journal of Biomechanics·D Mokhbi SoukaneJ P G Urban
Jul 17, 2007·European Spine Journal : Official Publication of the European Spine Society, the European Spinal Deformity Society, and the European Section of the Cervical Spine Research Society·Mauro AliniHans Joachim Wilke
Nov 13, 2008·Journal of Cell Communication and Signaling·W Mark Erwin
Nov 28, 2008·Developmental Dynamics : an Official Publication of the American Association of Anatomists·Kyung-Suk ChoiBrian D Harfe
Apr 1, 2009·Arthritis and Rheumatism·Thorsten GuehringJill P G Urban

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Citations

Sep 29, 2011·European Spine Journal : Official Publication of the European Spine Society, the European Spinal Deformity Society, and the European Section of the Cervical Spine Research Society·Benjamin Gantenbein-Ritter, Samantha C W Chan
Dec 3, 2010·Disease Models & Mechanisms·Lachlan J SmithDawn M Elliott
Nov 22, 2013·European Spine Journal : Official Publication of the European Spine Society, the European Spinal Deformity Society, and the European Section of the Cervical Spine Research Society·Esther Potier, Keita Ito
Apr 30, 2014·European Spine Journal : Official Publication of the European Spine Society, the European Spinal Deformity Society, and the European Section of the Cervical Spine Research Society·Ricardo Rodrigues-PintoJudith A Hoyland
Oct 29, 2014·Archives of Orthopaedic and Trauma Surgery·G W OmlorT Guehring
Jun 1, 2011·Cellular and Molecular Bioengineering·Jessica Czamanski SalvatierraC-Y Charles Huant
Jun 13, 2012·Journal of Anatomy·Girish PattappaSibylle Grad
Jul 19, 2013·Journal of Orthopaedic Research : Official Publication of the Orthopaedic Research Society·William AnderstJames Kang
Nov 29, 2013·Journal of Orthopaedic Research : Official Publication of the Orthopaedic Research Society·Hiroaki HirataKotaro Nishida
Feb 11, 2015·The Spine Journal : Official Journal of the North American Spine Society·Devina PurmessurJames C Iatridis
Mar 19, 2015·European Spine Journal : Official Publication of the European Spine Society, the European Spinal Deformity Society, and the European Section of the Cervical Spine Research Society·Dong-Dong XiaJun Tan
Dec 17, 2014·BMC Musculoskeletal Disorders·Benjamin GantenbeinSamantha C W Chan
Sep 7, 2014·Biochimica Et Biophysica Acta·Youssef YakkiouiYasin Temel
Nov 6, 2014·Journal of Spinal Disorders & Techniques·Robert A HartmanJames D Kang
Dec 30, 2014·The Spine Journal : Official Journal of the North American Spine Society·Takuto KurakawaKotaro Nishida
Dec 21, 2016·Clinical Anatomy : Official Journal of the American Association of Clinical Anatomists & the British Association of Clinical Anatomists·Malik GhannamR Shane Tubbs
Jan 18, 2014·Global Spine Journal·D PurmessurJ C Iatridis
Aug 31, 2017·Journal of Orthopaedic Research : Official Publication of the Orthopaedic Research Society·Paolo E Palacio-ManchenoJames C Iatridis
Apr 20, 2019·Frontiers in Bioengineering and Biotechnology·Lukas M JaworskiAlicia R Jackson
Oct 14, 2020·Journal of Orthopaedic Research : Official Publication of the Orthopaedic Research Society·Yutaro KandaKenichiro Kakutani
Jul 18, 2021·Experimental & Molecular Medicine·Yu SongCao Yang

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