Sensitivity of polymerase chain reaction for detection of bovine viral diarrhea virus in pooled serum samples and use of pooled polymerase chain reaction to determine prevalence of bovine viral diarrhea virus in auction market cattle

Journal of Veterinary Diagnostic Investigation : Official Publication of the American Association of Veterinary Laboratory Diagnosticians, Inc
Rebecca L SmithM Daniel Givens

Abstract

Two reverse transcription-nested polymerase chain reaction tests, 1 quantitative (qRT-nPCR) and 1 standard (RT-nPCR), were evaluated to assess sensitivity for detection of bovine viral diarrhea virus (BVDV) of a single positive serum sample in a pool of 30. The RT-nPCR and qRT-nPCR each detected 95 of 100 known positives. The RT-nPCR was used to estimate the prevalence of BVDV in adult beef cows. Serum samples were obtained from the US Department of Agriculture brucellosis testing laboratories in 3 Midwestern states. Samples originated from auction markets and private treaty sales throughout the 3 states. A total of 2,990 serum samples were collected and randomly pooled into 100 pools for testing. Two of the 100 pools of field samples were positive, and each positive pool had a single positive individual sample upon confirmation. The estimate of BVDV prevalence in adult cows in this study was 0.07%. This study estimates the diagnostic sensitivity of RT-nPCR for BVDV and confirms that it is a useful diagnostic tool for pools of 30 serum samples and that prevalence of BVDV in adult cattle from auction markets is low.

References

May 29, 2000·Journal of Veterinary Diagnostic Investigation : Official Publication of the American Association of Veterinary Laboratory Diagnosticians, Inc·C A Muñoz-ZanziS K Hietala
Nov 8, 2002·Biologicals : Journal of the International Association of Biological Standardization·Edgar StuderUrs Candrian
Jan 28, 2003·BioTechniques·Fred J DeGravesBernhard Kaltenboeck
Apr 16, 2003·American Journal of Veterinary Research·M Daniel GivensDavid A Stringfellow
Apr 6, 2004·The Veterinary Clinics of North America. Food Animal Practice·Daniel L Grooms
Feb 15, 2005·Journal of the American Veterinary Medical Association·Robert L LarsonBrad J White
Mar 4, 2005·Journal of the American Veterinary Medical Association·Guy H LoneraganRobert L Larson
Jan 24, 2006·Preventive Veterinary Medicine·Claudia Muñoz-ZanziWesley Johnson
Mar 29, 2006·Journal of Veterinary Diagnostic Investigation : Official Publication of the American Association of Veterinary Laboratory Diagnosticians, Inc·James A KennedyBarbara Powers

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Citations

Sep 24, 2013·The Veterinary Journal·Sasha R LanyonJoe Brownlie
Mar 15, 2011·Journal of Veterinary Diagnostic Investigation : Official Publication of the American Association of Veterinary Laboratory Diagnosticians, Inc·Jason S NickellMike W Sanderson
Nov 26, 2020·Brazilian Journal of Microbiology : [publication of the Brazilian Society for Microbiology]·Maximiliano J SpetterErika A González Altamiranda
Oct 10, 2021·Tropical Animal Health and Production·Juan Heberth Hernandez-MedranoWendela Wapenaar

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Methods Mentioned

BETA
PCR
biopsy

Software Mentioned

SAS

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Brucellosis is a bacterial infection caused by members of the genus brucella and remains one of the world's major zoonotic diseases. Discover the latest research on Brucellosis here.

Brucellosis

Brucellosis is a bacterial infection caused by members of the genus brucella and remains one of the world's major zoonotic diseases. Discover the latest research on Brucellosis here.

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