Sensitivity of two methods to detect Mycoplasma agalactiae in goat milk

Irish Veterinary Journal
J Tatay-DualdeJoaquín Amores

Abstract

Laboratory diagnostic techniques able to detect Mycoplasma agalactiae are essential in contagious agalactia in dairy goats. This study was designed: 1) to determine the detection limits of PCR and culture in goat milk samples, 2) to examine the effects of experimental conditions including the DNA extraction method, PCR technique and storage conditions (fresh versus frozen stored milk samples) on these methods and 3), to establish agreement between PCR and culture techniques using milk samples from goats with mastitis in commercial dairy herds. The study was conducted both on artificially inoculated and field samples. Our findings indicate that culture is able to detect M. agalactiae in goat milk at lower concentrations than PCR. Qualitative detection of M.agalactiae by culture and PCR was not affected by sample freezing, though the DNA extraction method used significantly affected the results of the different PCR protocols. When clinical samples were used, both techniques showed good agreement. The results from this study indicate that both culture and PCR are able to detect M. agalactiae in clinical goat mastitis samples. However, in bulk tank milk samples with presumably lower M. agalactiae concentrations, culture is recommen...Continue Reading

References

Oct 1, 1994·Journal of Veterinary Diagnostic Investigation : Official Publication of the American Association of Veterinary Laboratory Diagnosticians, Inc·H KindeR Petty
May 24, 2001·The Veterinary Record·M ThrusfieldK Frankena
Aug 23, 2005·Medical Mycology·P E Verweij
May 15, 2012·Journal of Microbiological Methods·Claire A M BeckerFlorence Tardy

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Citations

Apr 6, 2016·Theriogenology·Miranda Prats-van der HamÁngel Gómez-Martín
Sep 27, 2018·International Journal of Veterinary Science and Medicine·Amr El-SayedHugo Castañeda Vázquez

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