PMID: 9658208Jul 11, 1998Paper

Sensitivity to cisplatin and platinum-containing compounds of Schizosaccharomyces pombe rad mutants

Molecular Pharmacology
P PeregoS B Howell

Abstract

The role of genes that affect response to radiation in determining sensitivity to platinum-containing compounds was studied using a panel of 23 strains of the yeast Schizosaccharomyces pombe. The radiation-hypersensitive mutants all had the same genetic background and most of them contained mutations that disabled either cell cycle checkpoints or DNA repair. The tested platinum compounds included cisplatin and two complexes containing diaminocyclohexane (oxaliplatin and tetraplatin), two ammine/cyclohexylamine complexes with different orientation of the leaving groups (JM216 and JM335) and a multinuclear platinum complex (BBR 3464). The cytotoxic effect of the selected platinum complexes was evaluated by using a microtiter growth inhibition assay with a 48 hr exposure to drug. The mutants fell into three groups with respect to sensitivity to cisplatin: four mutants (rad2, -7, -11, -15) exhibited minimal change in sensitivity; fifteen mutants (rad4-6, -8-10, -12-14, -16-17, -19-21, and -22) were 5.1-21.7-fold hypersensitive; only rad1 and -3 mutants, defective in checkpoints, and rad18, defective in repair, displayed a marked hypersensitivity. None of the mutants demonstrated appreciable change in sensitivity to JM216 presumably...Continue Reading

References

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Citations

Dec 7, 2013·PloS One·Kanako HagiharaReiko Sugiura
May 13, 2003·International Journal of Cancer. Journal International Du Cancer·Paola PeregoFranco Zunino
Jun 24, 2010·The Journal of Antibiotics·Kellie JoyceArnold L Demain
Oct 5, 2001·Journal of the National Cancer Institute·T Fojo
Apr 21, 2001·European Journal of Cancer : Official Journal for European Organization for Research and Treatment of Cancer (EORTC) [and] European Association for Cancer Research (EACR)·T ServideiR Riccardi
Feb 9, 2000·Chemistry & Biology·Z Z ZdraveskiJ M Essigmann
Feb 14, 2006·Biochimica Et Biophysica Acta·Sabina C RighettiFranco Zunino
Jul 28, 2009·Journal of Experimental & Clinical Cancer Research : CR·Tadahiko NakamuraHideo Baba

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