PMID: 7520996Sep 1, 1994Paper

Sensitivity to nitrogen mustard in Saccharomyces cerevisiae is independently determined by regulated choline permease and DNA repair

Mutation Research
Z Y Li, M Brendel

Abstract

Sensitivity of yeast cells to the bifunctional alkylating agent nitrogen mustard (HN2) depends on two independently operating physiological mechanisms of cellular metabolism: dynamics of uptake of HN2 via choline permease, encoded in the gene HNM1/CTR, and repair of HN2-induced DNA damage. Uptake of choline and HN2 is impaired in mutant alleles of HNM1/CTR, leading to a HN2 hyper-resistant phenotype. Overexpression of HNM1/CTR leads to HN2 sensitivity higher than that of the wild type. While mutation and regulation of HNM1/CTR have pronounced effects on the cell's HN2 sensitivity, they do not interfere with repair of HN2-induced DNA damage, a process whose quality independently determines a yeast cell's sensitivity to HN2. Consequently, HNM1/CTR overexpression in an excision repair-deficient strain leads to extreme HN2 sensitivity whereas a mutational block of HNM1/CTR, in combination with excision proficiency, yields a HN2 hyper-resistant phenotype.

References

Oct 2, 1979·Molecular & General Genetics : MGG·R Fleer, M Brendel
Jul 1, 1992·Current Genetics·M Grey, M Brendel
Dec 1, 1990·Current Genetics·J A Henriques, M Brendel
Oct 25, 1988·Nucleic Acids Research·G Del SalC Schneider
Sep 12, 1973·Molecular & General Genetics : MGG·M Brendel, R H Haynes
Jan 1, 1983·Journal of Bacteriology·H ItoA Kimura
Jul 1, 1980·Journal of Bacteriology·K Hosaka, S Yamashita
Jan 1, 1981·Molecular & General Genetics : MGG·A RuhlandM Brendel

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