Sensitivity to Strain and Shear Stress of Isolated Mechanosensitive Enteric Neurons

Neuroscience
Eva M KuglerGemma Mazzuoli-Weber

Abstract

Within the enteric nervous system, the neurons in charge to control motility of the gastrointestinal tract reside in a particular location nestled between two perpendicular muscle layers which contract and relax. We used primary cultured myenteric neurons of male guinea pigs to study mechanosensitivity of enteric neurons in isolation. Ultrafast Neuroimaging with a voltage-sensitive dye technique was used to record neuronal activity in response to shear stress and strain. Strain was induced by locally deforming the elastic cell culture substrate next to a neuron. Measurements showed that substrate strain was mostly elongating cells. Shear stress was exerted by hydrodynamic forces in a microchannel. Both stimuli induced excitatory responses. Strain activated 14% of the stimulated myenteric neurons that responded with a spike frequency of 1.9 (0.7/3.2) Hz, whereas shear stress excited only a few neurons (5.6%) with a very low spike frequency of 0 (0/0.6) Hz. Thus, shear stress does not seem to be an adequate stimulus for mechanosensitive enteric neurons (MEN) while strain activates enteric neurons in a relevant manner. Analyzing the adaptation behavior of MEN showed that shear stress activated rapidly/slowly/ultraslowly adapting M...Continue Reading

Citations

Oct 17, 2018·Cell and Tissue Research·Gemma Mazzuoli-WeberMichael Schemann
Mar 11, 2020·Nature Reviews. Gastroenterology & Hepatology·Nick J Spencer, Hongzhen Hu
Aug 17, 2020·Scientific Reports·Anna Katharina FilzmayerGemma Mazzuoli-Weber
Feb 16, 2021·Frontiers in Neuroscience·Danli PengMelanie Stamp

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