Abstract
Rat/mouse T cell hybridoma-derived PC60 R55/R75 cells were used as a model to study tumour necrosis factor (TNF)-induced apoptosis. The role of ornithine decarboxylase (ODC) activity and polyamines in this process was investigated. In PC60 R55/R75 cells, TNF-induced ODC activity was completely suppressed by externally added spermine (Spm). TNF decreased the intracellular levels of the three polyamines Spm, spermidine (Spd) and putrescine (Put). A reduction of the intracellular [Spm] with methylglyoxal bis(quanyl hydrasone) (MGBG), CGP48644a, or bis(ethyl)norspermine (BENSpm), clearly sensitized the cells towards the apoptotic effect of TNF. Conversely, an increase in intracellular [Spm] with DFMO or externally added Spm reduced cellular sensitivity. Similar results were obtained after TNF treatment of the human cell lines Kym 39A6 (rhabdomyosarcoma), HeLaH21 (cervix carcinoma) and U937 (histocytoma) and after alphaFas treatment of HeLaH21, U937 and CEM-CM3 (human T cell line). These results suggest that a decrease of intracellular Spm levels rather then ODC activity per se is involved in the sensitization towards apoptosis induced by TNF or alphaFas.
Citations
Apr 30, 2008·Archives of Toxicology·José M MatésJavier Márquez
Feb 20, 2010·Amino Acids·G BjelakovićLj Bjelakovic
Sep 10, 2005·Apoptosis : an International Journal on Programmed Cell Death·M SchillerH M Lorenz
Oct 12, 2012·In Vitro Cellular & Developmental Biology. Animal·Gladys Chirino-GalindoMartín Palomar-Morales
Oct 30, 2001·Journal of Hepatology·G GardiniS Colombatto
Feb 25, 2000·The International Journal of Biochemistry & Cell Biology·J M Matés, F M Sánchez-Jiménez
Mar 21, 2001·The Biochemical Journal·C StefanelliA E Pegg
Oct 9, 2013·The Journal of Experimental Medicine·Xuan ZhouYi Zheng
Mar 25, 2006·Future Oncology·Maurice P Zeegers, Harry Ostrer
Aug 26, 2014·Molecular Pharmaceutics·Yu ZhuDavid Oupický
Jun 14, 2000·Cytokine·L C PenningP Vandenabeele
Jun 9, 2005·Stem Cells·Claudio MuscariClaudio Marcello Caldarera
Oct 6, 2005·Journal of Cellular and Molecular Medicine·Nikolaus Seiler, Francis Raul
Dec 20, 2008·Journal of Cellular Physiology·Ivana Stanic'Flavio Flamigni
Jan 31, 2008·Journal of Cellular Physiology·Ivana StanicFlavio Flamigni
Dec 11, 1999·Biochemical Pharmacology·A ToninelloM Mancon
Nov 16, 2004·Toxicology and Applied Pharmacology·Maria Elena FerioliSamuela Caimi
Sep 1, 2006·Environmental Toxicology and Pharmacology·Jin Sik KimIn Gyu Kim
Jun 12, 2001·Experimental Cell Research·C HegardtS M Oredsson
Aug 1, 2009·The International Journal of Biochemistry & Cell Biology·Kazuei Igarashi, Keiko Kashiwagi
Jul 5, 2005·The Journal of Biological Chemistry·Ramesh M RayLeonard R Johnson
Mar 11, 2000·American Journal of Physiology. Cell Physiology·R M RayL R Johnson
Apr 9, 2001·American Journal of Physiology. Gastrointestinal and Liver Physiology·L LiJ Y Wang
May 20, 2018·Science Immunology·Conor J KearneyJane Oliaro
Jul 11, 2000·Seminars in Cancer Biology·R G SchipperA A Verhofstad
Apr 11, 2006·Journal of Proteome Research·Claudia S AlgeMarius Ueffing